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• Long trebler phosphoramidite

Long trebler phosphoramidite

Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using a standard DNA synthesizer.

长高音亚磷酰胺是一种用于寡核苷酸合成的支化试剂，允许使用标准 DNA 合成仪合成支化的 DNA 结构。

After the condensation of the trebler, three DNA branches begin to grow simultaneously with each step of the synthesis. Deblock of this construct gives rise to DNA containing a branching point. One arm (stem) is attached to the branch point with its 5′-end, and other arms (branches) are attached with their 3′-ends. Reverse amidites can be used to prepare constructs with different branch orientations. Repetitive condensations of trebler results in formation of DNA dendrimers.

Trebler amidite can be used to attach several modifier amidites to the 5′-end of an oligonucleotide – for example, three biotin residues can be attached at once.

This amidite does not require any special handling. Recommended coupling time is 5 minutes. No changes to deblock conditions are required.

高音凝聚后，三个DNA分支开始随着合成的每一步同时生长。 该构建体的解封产生包含分支点的DNA。 一个臂（茎）以其 5′ 端连接到分支点，其他臂（分支）以其 3′ 端连接。 反向亚酰胺可用于制备具有不同分支方向的构建体。 高音重复缩合导致DNA树枝状大分子的形成。

Trebler 亚酰胺可用于将几个修饰亚酰胺连接到寡核苷酸的 5′ 端 – 例如，可以一次连接三个生物素残基。

这种亚酰胺不需要任何特殊处理。 推荐的耦合时间为 5 分钟。 不需要改变去块条件。