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- Cyanine5.5 phosphoramidite, 5′-terminal
Cyanine5.5 phosphoramidite, 5′-terminal
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 2680-100mg | 100 mg | – | 7 days |
| 2680-250mg | 250 mg | 290.00$ | 10 days |
| 2680-1g | 1 g | 950.00$ | in stock |
| 2680-10g | 10 g | 6750.00$ | 21 days |
This phosphoramidite can be used for the synthesis of 5′-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.
The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.
Cy5.5 是一种荧光团，在光谱的远红色范围内发射。 这种染料可用于多重 qPCR。 商用六通道 qPCR 仪器通常有一个用于 Cy5.5 的通道。
该亚磷酰胺可用于通过在寡核苷酸合成仪中直接标记来合成5′-标记的寡核苷酸。
与仲碳原子相连的亚磷酰胺官能团结构提供了额外的稳定性，可防止 Arbuzov 重排。 与衍生自伯醇的亚磷酰胺相比，这有助于在寡核苷酸合成仪中在较长的存储时间内保持偶联性能。
Absorption and emission spectra of Cyanine5.5
Cyanine5.5 phosphoramidite, 5′-terminal相关产品
Phosphate CPG 1000 solid support
Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.
磷酸盐修饰剂 CPG，用于固相合成含有 3 个磷酸基修饰的长（最多 120 聚体）寡核苷酸。
Cyanine5 phosphoramidite
Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.
Cy5 的亚磷酰胺衍生物，用于寡核苷酸合成。
FAM CPG 500, 6-isomer
Controlled pore glass for the synthesis of modified oligonucleotides containing 3′-fluorescein (FAM) modification.
用于合成含有 3′-荧光素 (FAM) 修饰的修饰寡核苷酸的可控孔玻璃。
General properties
| Appearance: | dark colored solid |
| Molecular weight: | 916.61 |
| Molecular formula: | C55H71N5ClO3P |
| Quality control: | NMR 1H, 31P, HPLC-MS (85%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 694 |
| ε, L⋅mol−1⋅cm−1: | 198000 |
| Emission maximum, nm: | 710 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.07 |
| CF280: | 0.03 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
