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- Cyanine5 phosphoramidite
Cyanine5 phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 1740-100mg | 100 mg | – | in stock |
| 1740-250mg | 250 mg | 290.00$ | 10 days |
| 1740-1g | 1 g | 950.00$ | in stock |
| 1740-10g | 10 g | 6750.00$ | 10 days |
Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.
This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5′-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent.
Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement – a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases stability of the reagent, maintaining efficient coupling over longer time.
Cy5 是 qPCR 中使用的染料之一。 在许多 qPCR 机器上都可以找到相应的过滤器集。
这种反应性衍生物可用于合成在 5′ 端具有花青 5 的 qPCR 探针，这是荧光团最典型的位置。 这是一种终止性非核苷试剂。
该试剂中的酰胺基与二级氧结合。 这为 Arbuzov 重排提供了额外的稳定性 – 这种反应会导致寡核苷酸合成器中溶液中的亚酰胺劣化。 这种分子设计增加了试剂的稳定性，在更长的时间内保持有效的耦合。
Absorption and emission spectra of Cyanine5
Cy5 phosphoramidite相关产品
FAM Phosphoramidite, 6-Isomer
Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.
用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。
Cyanine3 phosphoramidite 5′
Phosphoramidite for the synthesis of 5’-cyanine3-labeled oligonucleotides.
用于合成 5′-菁 3 标记寡核苷酸的亚磷酰胺。
Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
Catalyst for bioconjugate synthesis by Click Chemistry.
Click Chemistry 用于生物共轭合成的催化剂。
General properties
| Appearance: | dark colored solid |
| Molecular weight: | 816.49 |
| Molecular formula: | C47H67N5ClO3P |
| Quality control: | NMR 1H, 31P, HPLC-MS (80%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended
推荐 6 分钟耦合时间 |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
建议在 +4°C 或超温和保护基团下放置 48 小时； 24 小时在 rt 可能 |