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- FAM-11-UTP, 6-isomer
FAM-11-UTP, 6-isomer
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 2714-50nmol | 50 nmol | 190.00$ | 21 days |
| 2714-250nmol | 250 nmol | 750.00$ | 21 days |
| 2714-500nmol | 500 nmol | 1200.00$ | 21 days |
| 2714-1umol | 1 umol | please inquire | 21 days |
6-fluorescein (FAM) derivative of uridine triphosphate (UTP). FAM is a fluorophore with a high quantum yield. The maximum emission of the fluorescence is at a wavelength of 513 nm in the green spectrum range.
6-FAM-11-UTP can be used as a substrate for RNA-polymerases T7, T3 and SP6 during in vitro transcription. RNA-probes produced with this method can be used for fluorescence hybridization in situ, including multiplex, and for Northern blot. Fluorescently-labeled cRNA can be used for microarray-based gene expression profiling.
尿苷三磷酸 (UTP) 的 6-荧光素 (FAM) 衍生物。 FAM 是一种具有高量子产率的荧光团。 在绿色光谱范围内，荧光的最大发射波长为 513 nm。
6-FAM-11-UTP 可在体外转录过程中用作 RNA 聚合酶 T7、T3 和 SP6 的底物。 用这种方法产生的 RNA 探针可用于原位荧光杂交，包括多重荧光杂交和 Northern 印迹。 荧光标记的 cRNA 可用于基于微阵列的基因表达谱分析。
Absorption and emission spectra of FAM
FAM-11-UTP, 6-isomer相关产品
Amino-11-UTP
Uridine triphosphate labeled with amino group intended for indirect non-radioactive enzymatic RNA labeling.
用氨基标记的三磷酸尿苷，用于间接非放射性酶促 RNA 标记。
FAM-11-dUTP, 6-isomer
FAM-derivative of dUTP with green emission for DNA labeling.
用于 DNA 标记的具有绿色发射的 dUTP 的 FAM 衍生物。
Biotin-11-UTP
Biotinylated UTP for enzymatic RNA labeling.
用于酶促 RNA 标记的生物素化 UTP。
General properties
| Appearance: | yellow/orange solid 黄色/橙色固体 |
| Molecular weight: | 1029.48 |
| Molecular formula: | C39H39N4Li3O22P3 |
| IUPAC name: | ((2R,3S,4R,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | soluble in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 490 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 513 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.20 |
| CF280: | 0.17 |