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• dsSafe Gel Staining Solution 10,000×

dsSafe Gel Staining Solution 10,000×

The dsSafe dye is a fluorescent dye for nucleic acid staining in agarose and polyacrylamide gels. dsSafe is the best and safer alternative to ethidium bromide (EtBr); it was shown to be less toxic, non-mutagenic, and non-carcinogenic.

dsSafe allows nucleic acid visualization with sensitivity similar to that of ethidium bromide. Dye excitation can be performed with both conventional UV transilluminators and blue light transilluminators, with the latter ones being safer for the eyes and nucleic acid sample. When blue light is used for dsSafe excitation, background fluorescence is lower than in the case of ethidium bromide excitation with UV light. Excised DNA fragments for subsequent cloning are not exposed to damaging UV radiation, thus increasing cloning efficacy and reducing the frequency of adverse mutations caused by UV.

The spectrum of the DNA-dye complex has two excitation maxima [in the UV (280 nm) and blue ranges (502 nm)] and one emission maximum in the green range (~530 nm). Available as 10,000× concentrate in DMSO. To stain the gel, add the dye to melted agarose solution* or stain the gel after gel electrophoresis in TAE/TBE buffer for 10–20 minutes.

dsSafe 染料是一种荧光染料，用于琼脂糖和聚丙烯酰胺凝胶中的核酸染色。 dsSafe 是溴化乙锭 (EtBr) 的最佳和更安全的替代品；它被证明是毒性较小、非致突变性和非致癌性的。

dsSafe 允许核酸可视化，其灵敏度与溴化乙锭相似。染料激发可以使用传统的紫外透射仪和蓝光透射仪进行，后者对眼睛和核酸样品更安全。当蓝光用于 dsSafe 激发时，背景荧光低于用紫外光激发溴化乙锭的情况。用于后续克隆的切除 DNA 片段不会暴露在有害的紫外线辐射下，从而提高克隆效率并降低紫外线引起的不良突变频率。

DNA-染料复合物的光谱有两个激发最大值 [在 UV (280 nm) 和蓝色范围 (502 nm)] 和一个发射最大值在绿色范围 (~530 nm)。以 DMSO 中的 10,000 倍浓缩物形式提供。要对凝胶进行染色，请将染料添加到融化的琼脂糖溶液中*，或在凝胶电泳后在 TAE/TBE 缓冲液中染色 10-20 分钟。

Key dye advantages主要染料优势:

• Non-mutagenic, non-carcinogenic, far less toxic dye for DNA and RNA staining用于 DNA 和 RNA 染色的非诱变、非致癌、毒性低得多的染料
• High sensitivity identical to that of EtBr与 EtBr 相同的高灵敏度
• Excitation both with UV and blue light紫外光和蓝光激发
• Easy to use: the dye can be added to melted agarose solution or the gel can be stained within 10 to 20 minutes after gel electrophoresis completion
• 易于使用：染料可添加到融化的琼脂糖溶液中或凝胶电泳完成后 10 至 20 分钟内染色
• Can be used for all routine laboratory procedures requiring nucleic acid visualization可用于所有需要核酸可视化的常规实验室程序
• Does not require special storage or disposal conditions不需要特殊的存储或处置条件

For nucleic acid gel staining, we also offer dsGreen Gel Staining Solution with higher sensitivity to DNA . However, in contrast with dsSafe, gel staining with dsGreen Gel Staining Solution is recommended only after the completion of gel electrophoresis.

对于核酸凝胶染色，我们还提供对 DNA 具有更高灵敏度的 dsGreen 凝胶染色溶液。 然而，与 dsSafe 相比，建议仅在完成凝胶电泳后使用 dsGreen 凝胶染色溶液进行凝胶染色。

*Similarly to ethidium bromide, dsSafe when added in melted agarose solution can slightly reduce the mobility of nucleic acid sample in the gel.

*与溴化乙锭类似，dsSafe 在融化的琼脂糖溶液中添加时会略微降低核酸样品在凝胶中的迁移率。