- Products
- DNA
- PCR/qPCR
- dsGreen for Real-Time PCR, 100×
dsGreen for Real-Time PCR, 100×
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 11010 | 100 uL | 39.00$ | in stock |
| 41010 | 1 mL | 310.00$ | in stock |
| 51010 | 1.5 mL | 450.00$ | in stock |
| 61010 | 2 mL | 498.00$ | in stock |
| 71010 | 5 mL | 845.00$ | in stock |
| 81010 | 10 mL | 1190.00$ | in stock |
| 91010 | 50 mL | 4450.00$ | in stock |
dsGreen is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
dsGreen 是一种非常灵敏的 dsDNA 检测染料。 dsDNA 的高灵敏度和高选择性允许使用 dsGreen 作为 qPCR 的通用 dsDNA 检测试剂。 无需使用标记探针来检测 dsGreen 的扩增——未标记的引物就足够了。
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
与 Lumiprobe 提供的其他用于凝胶染色的 dsGreen 制剂不同，该制剂专门设计用于实时 PCR 实验。 具体特点是：
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
- 染料浓度针对 qPCR 进行了优化，并仔细调整了批次间可重复的结果
- PCR tested preparation – quality guaranteed
- PCR 检测制剂 – 质量保证
- Low fluorescence background – high fluorescence intensity gain
- 低荧光背景 – 高荧光强度增益
qPCR curves with dsGreen
Excitation and emission spectra of dsDNA complex with dsGreen
相关产品
JOE phosphoramidite, 5-isomer
JOE xanthene dye phosphoramidite for oligo synthesis. Absorption and emission spectra for JOE are located between FAM and TAMRA channels.
JOE 呫吨染料亚磷酰胺用于寡核苷酸合成。 JOE 的吸收和发射光谱位于 FAM 和 TAMRA 通道之间。
Sulfo-Cyanine3 dUTP
Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3. The nucleotide contains a long linker that allows to incorporate the dye efficiently into DNA strand by various enzymes including Taq polymerase.
用于用磺基花青标记 DNA 的荧光 dNTP。 核苷酸包含一个长接头，允许通过包括 Taq 聚合酶在内的各种酶将染料有效地掺入 DNA 链中。
TAMRA azide, 5-isomer
TAMRA fluorescent dye azide for Click chemistry.
用于点击化学的 TAMRA 荧光染料叠氮化物。
General properties
| Appearance: | light orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 454 |
| ε, L⋅mol−1⋅cm−1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0,8 |
相关文献：
- Carter, J.G.; Iturbe, L.O.; Duprey, J.-L.H.A.; Carter, I.R.; Southern, C.D.; Rana, M.; Bosworth, A. Sub-5-minute Detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR. medRxiv, preprint. doi: 10.1101/2020.12.31.20248236
- Morozov, V.N.; Klimovich, M.A.; Kolyvanova, M.A.; Dement’eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Interaction of Gold Nanoparticles with Cyanine Dyes in Cholesteric DNA Submicroparticles. High Energy Chemistry, 2021, 55(5), 341–348. doi: 10.1134/S0018143921050088
- Carter, J.G.; Iturbe, L.O.; Duprey, J.-L.H.A.; Carter, I.R.; Southern, C.D.; Rana, M.; Whalley, C.M.; Bosworth, A.; Beggs, A.D.; Hicks, M.R.; Tucker, J.H.R.; Dafforn, T.R. Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription{textendash}free exponential amplification reaction, RTF-EXPAR. Proceedings of the National Academy of Sciences of the U.S.A., 2021, 118(35), e2100347118. doi: 10.1073/pnas.2100347118
- Fang, Y.; Coulter, J.A.; Wu, J.; Liu, L.; Li, X.; Dong, Y.; Ma, L.; Pu, Y.; Sun, B.; Niu, Z.; Jin, J.; Zhao, Y.; Mi, W.; Xu, Y.; Sun, W. Identification of differentially expressed genes involved in amino acid and lipid accumulation of winter turnip rape (Brassica rapa L.) in response to cold stress. PloS ONE, 2021, 16(2), e0245494. doi: 10.1371/journal.pone.0245494