dsGreen Gel Staining Solution, 10000×

dsGreen Gel Staining Solution, 10000×,dsGreen 是一种灵敏的 dsDNA 结合染料，可用于琼脂糖和聚丙烯酰胺凝胶中的常规 DNA 检测。 还提供 qPCR 级试剂。与溴化乙锭不同，dsGreen 对双链 DNA 具有高度选择性，危害更小，灵敏度更高。

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  • dsGreen Gel Staining Solution, 10000×

dsGreen Gel Staining Solution, 10000×

标签：PCR and qPCR,DNA
货号 规格 价格 货期
A0010 50 uL 29.00$ in stock
10010 0.5 mL 100.00$ in stock
20010 1 mL 180.00$ in stock
dsGreen is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.

Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.

dsGreen 是一种灵敏的 dsDNA 结合染料，可用于琼脂糖和聚丙烯酰胺凝胶中的常规 DNA 检测。 还提供 qPCR 级试剂。
与溴化乙锭不同，dsGreen 对双链 DNA 具有高度选择性，危害更小，灵敏度更高。

Comparison between ethidium bromide and dsGreen

溴化乙锭和dsGreen的比较

Feature Ethidium Bromide dsGreen
Fluorescence Red (615 nm) Green (524 nm)
Excitation maximum 302 nm 454 nm
Excitation light source UV only Blue light or UV
Sensitivity 2 ng / band (dsDNA)
100 ng / band (RNA)		 0.08 ng / band (dsDNA)
1–2 ng / band (oligonucleotides)
Health hazard High Low

 

Excitation and emission spectra of dsDNA complex with dsGreen

dsGreen Gel Staining Solution, 10000×

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General properties

Appearance: orange solution
Quality control: NMR 1H, HPLC-MS (95%), functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 454
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0.8

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  1. Barrios-Hernández, M.L.; Bettinelli, C.; Mora-Cabrera, K.; Vanegas-Camero, M.-C.; Garcia, H.; van de Vossenberg, J.; Prats, D.; Brdjanovic, D.; van Loosdrecht, M.C.M.; Hooijmans, C.M. Unravelling the removal mechanisms of bacterial and viral surrogates in aerobic granular sludge systems. Water Research, 2021, 195, 116992. doi: 10.1016/j.watres.2021.116992
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  4. Kirkham, C.M.; Scott, J.N.F.; Wang, X.; Smith, A.L.; Kupinski, A.P.; Ford, A.M.; Westhead, D.R.; Stockley, P.G.; Tuma, R.; Boyes, J. Cut-and-Run: A Distinct Mechanism by which V(D)J Recombination Causes Genome Instability. Molecular Cell, 2019, 74(3), 584–597.e9. doi: 10.1016/j.molcel.2019.02.025