Pico488 dsDNA quantification reagent, 200x solution in DMSO

Pico488 dsDNA quantification reagent, 200x solution in DMSO,Pico488 是一种 DNA 结合苯并噻唑染料，其结构与 PicoGreen® 相同。 该试剂是 DMSO 中的 200 倍溶液。 该染料是一种特定于双链 DNA (dsDNA) 的荧光传感器。 它在荧光素通道中的吸收和发射可以很容易地使用比色皿荧光计、孔板读数器和专用 DNA 测量荧光计（如 Qubit®）检测到。

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  • Pico488 dsDNA quantification reagent, 200x solution in DMSO

Pico488 dsDNA quantification reagent, 200x solution in DMSO

标签：PCR and qPCR, Nucleic acid quantification
货号 Quantity 价格 货期
12010 100 uL 39.00$ in stock
42010 1 mL 290.00$ in stock
62010 25 mL please inquire in stock
92010 50 mL please inquire in stock

Pico488 is a DNA binding benzothiazole dye whose structure is identical to PicoGreen®. This reagent is a 200× solution in DMSO. The dye is a fluorescent sensor that is specific for double stranded DNA (dsDNA). Its absorption and emission in the fluorescein channel can be easily detected using cuvette fluorometers, well plate readers, and by dedicated DNA measurement fluorometers like Qubit®.

The dye exhibits a linear response of fluorescence to dsDNA concentrations in the range of low pg/mL to high ng/mL. The ready kit including quantification dye is also available.

Pico488 是一种 DNA 结合苯并噻唑染料，其结构与 PicoGreen® 相同。 该试剂是 DMSO 中的 200 倍溶液。 该染料是一种特定于双链 DNA (dsDNA) 的荧光传感器。 它在荧光素通道中的吸收和发射可以很容易地使用比色皿荧光计、孔板读数器和专用 DNA 测量荧光计（如 Qubit®）检测到。

该染料在低 pg/mL 至高 ng/mL 范围内对 dsDNA 浓度表现出荧光的线性响应。 还提供包含定量染料的现成试剂盒。

Pico488 (Pico Green) excitation and emission spectra

Pico488 dsDNA quantification reagent, 200x solution in DMSO

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Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.

Cy5 马来酰亚胺与硫醇反应生成染料标记的偶联物。

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

胺反应性 Cy5 活化酯，用于标记蛋白质、肽和其他分子。

BDP TR NHS ester

BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.

BDP TR 是一种硼二吡咯亚甲基染料。 具有高量子产率的明亮且光稳定的荧光团。

General properties

Appearance: orange solution
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 503
Emission maximum, nm: 526

相关文献：

  1. Norred, S.E.; Dabbs, R.M.; Chauhan, G.; Caveney, P.M.; Collier, C.P.; Abel, S.M.; Simpson, M.L. Synergistic interactions between confinement and macromolecular crowding spatially order transcription and translation in cell-free expression. bioRxiv, preprint. doi: 10.1101/445544
  2. Morozov, V.N.; Klimovich, M.A.; Kolyvanova, M.A.; Dement’eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Interaction of Gold Nanoparticles with Cyanine Dyes in Cholesteric DNA Submicroparticles. High Energy Chemistry, 2021, 55(5), 341–348. doi: 10.1134/S0018143921050088
  3. Wiktor, J.; Gynnå, A.H.; Leroy, P.; Larsson, J.; Coceano, G.; Testa, I.; Elf, J. RecA finds homologous DNA by reduced dimensionality search. Nature, 2021, 597(7876), 426–429. doi: 10.1038/s41586-021-03877-6
  4. Morozov, V.N.; Kolyvanova, M.A.; Dement’eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Comparison of quenching efficacy of SYBR green I and picoGreen fluorescence by ultrasmall gold nanoparticles in isotropic and liquid-crystalline DNA systems. Journal of Molecular Liquids, 2021, 321, 114751. doi: 10.1016/j.molliq.2020.114751
PicoGreen® and Qubit® are trademarks of Molecular Probes, Inc.