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- Cyanine3.5 DBCO
Cyanine3.5 DBCO
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| A20F0 | 1 mg | 110.00$ | in stock |
| B20F0 | 5 mg | 210.00$ | in stock |
| C20F0 | 10 mg | 310.00$ | in stock |
| D20F0 | 25 mg | 410.00$ | in stock |
| E20F0 | 50 mg | 695.00$ | in stock |
| F20F0 | 100 mg | 1190.00$ | in stock |
Cyanine3.5 is a bright and photostable fluorophore, which is similar in its spectral characteristics to Cy3.5™ dye. Cyanine3.5 DBCO fluorescence maximum is at 604 nm and in the orange-red spectrum range.
Cyanine3.5 fluorescence is pH insensitive in the range from 4 to 10 and can be detected in the spectrum range with a low level of autofluorescence of biological samples.
Cyanine3.5 DBCO (dibenzocyclooctyne) easily forms conjugates with azide derivatives of biomolecules in copper-free Click Chemistry. The process runs without catalysts or increased temperature, resulting in stable triazoles.
Conjugates of biomolecules with Cyanine3.5 can be used for various microscopy assays, including FRET-microscopy, and in proteomics.
Cyanine3.5 是一种明亮且光稳定的荧光团，其光谱特性与 Cy3.5™ 染料相似。 Cyanine3.5 DBCO 荧光最大值在 604 nm 处，在橙红色光谱范围内。
Cyanine3.5 荧光在 4 到 10 的范围内对 pH 不敏感，并且可以在生物样品的低水平自发荧光的光谱范围内检测到。
Cyanine3.5 DBCO（二苯并环辛炔）在无铜点击化学中很容易与生物分子的叠氮化物衍生物形成共轭物。 该过程在没有催化剂或升高温度的情况下运行，从而产生稳定的三唑。
生物分子与 Cyanine3.5 的偶联物可用于各种显微镜分析，包括 FRET 显微镜和蛋白质组学。
Absorption and emission spectra of Cyanine3.5
Cyanine3.5 DBCO相关产品
Cyanine5 DBCO
Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper free Click chemistry.
用于无铜点击化学的具有二苯并环辛炔 (DBCO) 官能团的 Cy5 染料。
Cyanine3 DBCO
Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper free Click chemistry.
Cy3 dibenzocyclooctyne (DBCO, or ADIBO) 是一种用于无铜点击化学的环炔烃染料衍生物。
sulfo-Cyanine5.5 DBCO
Sulfo-Cyanine5.5 DBCO is a far-red, water-soluble reagent for copper-free reaction with azides.
Sulfo-Cy5.5 DBCO 是一种远红色的水溶性试剂，用于与叠氮化物的无铜反应。
General properties
| Appearance: | dark violet solid 深紫色固体 |
| Molecular weight: | 944.95 |
| Molecular formula: | C59H61N4BF4O2 |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 591 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 604 |
| Fluorescence quantum yield: | 0.35 |
| CF260: | 0.29 |
| CF280: | 0.22 |