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• BDP 576/589 tetrazine

BDP 576/589 tetrazine

Because of its relatively long excited-state lifetime (about 5 nanoseconds), BDP 576/589 dye can be used in various methods based on measuring fluorescence lifetime. Similarly to other dyes of the BDP family, BDP 576/589 has strong hydrophobic properties and is suitable for labeling non-polar and lypophilic biomolecules and their subsequent visualization by fluorescent microscopy, including two-photon microscopy.

This reagent is a tetrazine derivative that can be conjugated with various strained dienophiles such as trans-cyclooctenes and cyclopropenes. This reaction (TCO ligation) is considered to be one of the best bioconjugation reactions because it is very fast and selective in physiological conditions and does not require additional catalyzers, so it is not toxic in vitro and in vivo.

由于其相对较长的激发态寿命（约 5 纳秒），BDP 576/589 染料可用于基于测量荧光寿命的各种方法。 与 BDP 家族的其他染料类似，BDP 576/589 具有很强的疏水性，适用于标记非极性和亲液性生物分子，并随后通过荧光显微镜（包括双光子显微镜）对其进行可视化。

该试剂是一种四嗪衍生物，可与各种应变亲双烯体（例如反式环辛烯和环丙烯）结合。 该反应（TCO 连接）被认为是最好的生物偶联反应之一，因为它在生理条件下非常快速且具有选择性，并且不需要额外的催化剂，因此在体外和体内都没有毒性。