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• Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 is a sulfated derivative of Cyanine3 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this yellow-orange fluorescent dye is an analog of Cy™3.

Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with Sulfo-Cyanine3 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with Sulfo-Cyanine3 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

Sulfo-Cy3 是 Cy3 染料的硫酸化衍生物，由于其结构中有两个带负电荷的磺基，因此可很好地溶于水。根据其光谱特性，这种橙黄色荧光染料是 Cy™3 的类似物。

酰肼有效地与醛和酮反应生成腙，因此该化合物可用于与生物分子的羰基衍生物缀合。

反应在水性条件下进行，这在处理抗体和蛋白质时很重要。糖基化蛋白质和抗体中的糖中的 Cys-二醇基团可被氧化成二醛，蛋白质中的半胱氨酸可被酶转化为甲酰基甘油（即与磺基花青 3 酰肼结合的反应基团）。蛋白质和肽中的天冬氨酸和谷氨酸的羧基也可以在活化剂存在的情况下与磺基花青 3 酰肼结合：碳二亚胺 (EDAC) 或甲基吗啉 (DMTMM) 衍生物。