Sulfo-Cyanine5 maleimide

Sulfo-Cyanine5 maleimide,水溶性、亲水性磺基-Cyanine5 马来酰亚胺（Cy5® 马来酰亚胺类似物）。 我们推荐该产品用于蛋白质标记，包括标记抗体作为 Cy5® 马来酰亚胺的完美替代品。 通过凝胶过滤、旋转柱纯化、透析、电泳或色谱法，可以很容易地将标记的蛋白质与未反应的染料分离。

  • Products
  • Reactive dyes
  • Dye maleimides
  • Sulfo-Cyanine5 maleimide

Sulfo-Cyanine5 maleimide

标签：sulfo-Cyanine5, Cy5,Maleimides, Microscopy
货号 规格 价格 货期
13380 1 mg 110.00$ in stock
23380 5 mg 290.00$ in stock
43380 25 mg 690.00$ in stock
53380 50 mg 1270.00$ in stock
63380 100 mg 1990.00$ in stock

A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.

An analog without sulfo groups, Cyanine5 maleimide, is also available.

水溶性、亲水性磺基-Cyanine5 马来酰亚胺（Cy5® 马来酰亚胺类似物）。 我们推荐该产品用于蛋白质标记，包括标记抗体作为 Cy5® 马来酰亚胺的完美替代品。 通过凝胶过滤、旋转柱纯化、透析、电泳或色谱法，可以很容易地将标记的蛋白质与未反应的染料分离。

也可使用不含磺基的类似物 Cyanine5 马来酰亚胺。

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 maleimide

Sulfo-Cyanine5 maleimide相关产品

TAMRA azide, 6-isomer

Pure 6-isomer of tetramethylrhodamine (TAMRA) dye, azide derivative for copper-catalyzed and copper-free Click chemistry reactions with alkynes.

纯 6 异构体的四甲基罗丹明 (TAMRA) 染料，叠氮化物衍生物，用于与炔烃的铜催化和无铜点击化学反应。

ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

用于胺标记的纯异构体 6-ROX NHS 酯。

Sulfo-Cyanine5 amine

Sulfonated Cyanine5 amine derivative, for the conjugation with electrophiles, and enzymatic transamination labeling.

磺化的 Cy5 胺衍生物，用于与亲电试剂偶联和酶促转氨标记。

General properties

Appearance: dark blue powder  深蓝色粉末
Molecular weight: 803.00
CAS number: 2242791-82-6
Molecular formula: C38H43KN4O9S2
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

相关文献：

  1. Ast, J.; Novak, A.N.; Podewin, T.; Fine, N.H.F.; Jones, B.; Tomas, A.; Birke, R.; Roßmann, K.; Mathes, B:; Eichhorst, J.; Lehmann, M.; Linnemann, A.K.; Hodson, D.J.; Broichhagen, J. An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo. ChemRxiv, preprint. doi: 10.33774/chemrxiv-2021-7rngq
  2. Delvaux, N.A.; Mathew, B.; Rice, K.G. Fluorescent Labeling of Plasmid DNA for Gene Delivery: Implications of Dye Hydrophobicity on Labeling Efficiencies and Nanoparticle Size. Analytical Biochemistry, in press. doi: 10.1016/j.ab.2020.113895
  3. Bauer, B.W.; Davidson, I.F.; Canena, D.; Wutz, G.; Tang, W.; Litos, G.; Horn, S.; Hinterdorfer, P.; Peters, J.-M. Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism. Cell, 2021, 184(21), 5448–5464.e22. doi: 10.1016/j.cell.2021.09.016
  4. Hurley, C.M.; Kraut, D.A. Determination of Proteasomal Unfolding Ability. Methods in Molecular Biology, 2021, 2365, 217–244. doi: 10.1007/978-1-0716-1665-9_12
Cy® is a trademark of GE Healthcare.