ROX maleimide, 5-isomer

ROX maleimide, 5-isomer,ROX 是一种明亮的罗丹明染料,最大发射波长约为 580 nm。 它广泛用于标记生物分子。 与其他罗丹明染料一样,它有两种异构体,5-ROX 和 6-ROX。 尽管这些异构体具有几乎相同的光谱特性,但需要将它们分离以避免在 HPLC 或电泳中标记产物加倍。

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  • ROX maleimide, 5-isomer

ROX maleimide, 5-isomer

标签:ROX (Rhodamine X, Rhodamine 101), Maleimides
货号 规格 价格 货期
11280 1 mg 110.00$ in stock
21280 5 mg 210.00$ in stock
41280 25 mg 410.00$ in stock
51280 50 mg 695.00$ in stock
61280 100 mg 1190.00$ in stock

ROX is a bright rhodamine dye with emission maximum around 580 nm. It is widely used for the labeling of biomolecules. As other rhodamine dyes, it comes as two isomers, 5-ROX and 6-ROX. Although these isomers have almost identical spectral properties, they need to be separated to avoid doubling of labeled products on HPLC or electrophoresis.

This derivative contains thiol-reactive maleimide moiety for the attachment of ROX fluorophore to SH groups, such as cystein residues in proteins and peptides.

ROX 是一种明亮的罗丹明染料,最大发射波长约为 580 nm。 它广泛用于标记生物分子。 与其他罗丹明染料一样,它有两种异构体,5-ROX 和 6-ROX。 尽管这些异构体具有几乎相同的光谱特性,但需要将它们分离以避免在 HPLC 或电泳中标记产物加倍。

该衍生物含有硫醇反应性马来酰亚胺部分,用于将 ROX 荧光团连接到 SH 基团,例如蛋白质和肽中的半胱氨酸残基。

Absorption and emission spectra of ROX

ROX maleimide, 5-isomer

ROX maleimide, 5-isomer相关文献

BDP R6G maleimide

BDP R6G is a borondipyrromethene dye tuned to R6G (rhodamine 6G) channel. This is a thiol reactive maleimide for the labeling of protein cysteine residues, and other thiols.

BDP R6G 是一种调谐到 R6G(罗丹明 6G)通道的硼二吡咯亚甲基染料。 这是一种硫醇反应性马来酰亚胺,用于标记蛋白质半胱氨酸残基和其他硫醇。

Sulfo-Cyanine7 maleimide

Water soluble near infrared dye maleimide for the labeling of proteins and peptides.

水溶性近红外染料马来酰亚胺,用于标记蛋白质和肽。

FAM maleimide, 6-isomer

Fluorescein (FAM) maleimide is a thiol reactive dye derived from isomerically pure 6-FAM. Fluorescein is a bright dye that is compatible with various fluorescence measurement instruments.

荧光素 (FAM) 马来酰亚胺是一种衍生自异构纯 6-FAM 的硫醇活性染料。 荧光素是一种与各种荧光测量仪器兼容的明亮染料。

General properties

Appearance: red powder  红色粉末
Mass spec M+ increment: 656.3
Molecular weight: 656.73
Molecular formula: C39H36N4O6
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.00