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• Sulfo-Cyanine3.5 azide

Sulfo-Cyanine3.5 azide

Sulfo-Cyanine3.5 is a fluorescent dye with absorption and emission spectra between those of Cyanine3 and Cyanine5. Because of its spectral characteristics, this fluorophore is widely used in BRET and FRET experiments both as a pair donor and acceptor.

Sulfo-Cyanine3.5 is a sulfonated derivative of Cyanine3.5 dye because it contains four additional sulfo groups, which ensures high water solubility of the dye and its conjugates. This allows working with this reagent in aqueous solutions without adding organic solvents.

This reagent is an azide derivative of sulfo-Cyanine3.5 dye for Click Chemistry reactions. The azide group allows conjugating the fluorophore with molecules containing alkyne or cycloalkyne groups, including various biomolecules, small molecules, and polymers, using copper-catalyzed Click chemistry and copper-free spAAC reaction.

Sulfo-Cyanine3.5 是一种荧光染料，其吸收和发射光谱介于 Cy3 和 Cy5 之间。 由于其光谱特性，这种荧光团广泛用于 BRET 和 FRET 实验，既可作为供体对，也可作为受体。

Sulfo-Cyanine3.5 是 Cyanine3.5 染料的磺化衍生物，因为它含有四个额外的磺基，可确保染料及其结合物的高水溶性。 这允许在不添加有机溶剂的情况下在水溶液中使用该试剂。

该试剂是用于 Click Chemistry 反应的磺基-Cyanine3.5 染料的叠氮化物衍生物。 叠氮基允许使用铜催化的点击化学和无铜 spAAC 反应将荧光团与含有炔烃或环炔烃基团的分子（包括各种生物分子、小分子和聚合物）缀合。