lumiprobe -Coumarin 343 X NHS ester

Coumarin 343 X NHS ester,蓝色发射香豆素 343 染料可与荧光素 (FAM) 形成 FRET 对。 胺反应形式,活化的 NHS 酯。

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  • Coumarin 343 X NHS ester

Coumarin 343 X NHS ester

标签:NHS esters Coumarin 343 Protein labeling
货号 规格 价格 货期
12620 1 mg 110.00$ 5 days
22620 5 mg 210.00$ in stock
42620 25 mg 410.00$ in stock
52620 50 mg 695.00$ in stock
62620 100 mg 1190.00$ in stock

Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.

This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.

This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.

蓝色发射香豆素 343 染料可与荧光素 (FAM) 形成 FRET 对。 胺反应形式,活化的 NHS 酯。

这种胺反应性 NHS 酯在荧光团和反应性基团之间含有氨基己酰基接头。 这种接头在荧光团和被标记的目标分子之间提供了更好的溶解度和空间分离。

该试剂可用于设计以 FAM 为受体的基于 FRET 的分析,并构建收集蓝光能量的系统。

Coumarin 343 X NHS ester Coumarin 343 X NHS ester Coumarin 343 X NHS ester Coumarin 343 X NHS ester

Absorption and emission spectra of Coumarin 343

Coumarin 343 X NHS ester

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AF568 衍生物,用于用游离胺标记蛋白质和其他高分子量和低分子量配体。

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水溶性磺基-Cyanine3 染料四嗪衍生物,用于用反式环辛烯进行 TCO 连接标记。

BDP 581/591 carboxylic acid

BDP 581/591 is a multi purpose fluorophore, that can also be used to probe reactive oxygen species (ROS). This is a carboxylic acid derivative with a free COOH group.

BDP 581/591 是一种多用途荧光团,也可用于探测活性氧 (ROS)。 这是一种带有游离 COOH 基团的羧酸衍生物。

General properties

Appearance: yellow solid
Molecular weight: 495.52
CAS number: 946123-12-2
Molecular formula: C26H29N3O7
Solubility: good in DCM, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 437
ε, L⋅mol−1⋅cm−1: 39000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63
CF260: 0.29
CF280: 0.24

相关文献:

  1. Zheng, L.; Zhao, H.; Han, Y.; Qian, H.; Vukovic, L.; Mecinović, J.; Král, P.; Huck, W.T.S. Catalytic transport of molecular cargo using diffusive binding along a polymer track. Nature Chemistry, 2019, 11(4), 359–366. doi: 10.1038/s41557-018-0204-7