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• Reactive dyes
• Dye NHS esters
• AF594 NHS ester, 5-isomer

AF594 NHS ester, 5-isomer

AF594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is commonly used for flow cytometry and fluorescent microscopy.
AF594是一种明亮的水溶性染料，对4到10范围内的pH变化不敏感。这种红色荧光染料通常用于流式细胞术和荧光显微镜。

AF594 NHS ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9.
AF594 NHS酯用于标记蛋白质、肽、抗体和任何含有-NH2基团的分子（如氨基修饰的寡核苷酸）；它导致在染料和目标分子之间形成稳定的酰胺键。在pH值为7到9时，共轭反应的效果最好。

AF594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.
AF594可用于高摩尔染料蛋白质比的蛋白质标记。由此产生的具有高度标记（DOL）的共轭物不会表现出明显的荧光猝灭。相比之下，共轭物具有更亮的荧光，这允许增加标记产品的最低检测限。