- Products
- Reactive dyes
- Dye NHS esters
- AF488 NHS ester
AF488 NHS ester
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 11820 | 1 mg | 110.00$ | in stock |
| 21820 | 5 mg | 290.00$ | in stock |
| 41820 | 25 mg | 690.00$ | in stock |
| 51820 | 50 mg | 1270.00$ | in stock |
| 61820 | 100 mg | 1990.00$ | in stock |
AF488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.
AF488是一种明亮且耐光的染料。由于其高亲水性，这是敏感蛋白质和抗体标记的首选染料。这种染料适用于许多苛刻的应用，包括显微镜。
From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties.
从化学角度来看，AF488是磺化罗丹明染料罗丹明110（R110）。与其他罗丹明类似，它可以作为5-和6-异构体，具有几乎相同的光物理性质。
The isomers need to be separated though – otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.
但是，异构体需要分离——否则，使用混合异构体染料可能会在标记产品的HPLC或电泳分离过程中导致双峰。本品为同分异构体纯5-AF488。
This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.
这种NHS酯是一种胺活性染料，它可以标记蛋白质、肽、氨基修饰低聚物和其他目标分子中的胺基。
Absorption and emission spectra of AF488
AF488 NHS ester 相关产品
DMF (dimethylformamide), labeling grade
Dry high purity amine free solvent for NHS ester labeling.
干燥高纯度无胺溶剂，用于NHS酯标记。
Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
胺反应性菁5活化酯，用于标记蛋白质、肽和其他分子。
Cyanine5.5 DBCO
Cyanine5.5 is a far-red / NIR emitting dye that is useful for bioimaging studies. Cyanine5.5 DBCO is a derivative of the dye with a cycloalkyne moiety for Copper free Click chemistry.
氰基5。5是一种远红色/近红外发光染料，可用于生物成像研究。氰基5。5 DBCO是该染料的衍生物，具有无铜点击化学的环炔部分。
General properties
| Appearance: | dark orange solid |
| Molecular weight: | 732.74 |
| Molecular formula: | C31H32N4O13S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid) |
| Storage conditions: | Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |