BAPTA-AM试剂货号：B018

BAPTA-AM是BAPTA羧基的乙酰氧基甲基酯化产物，可以很容易地吸收到细胞中。 该酯被细胞内酯酶分解，显示出主体在细胞中作为BAPTA的功能。 生成的BAPTA保留在细胞内部。

1) R. Y. Tsien, “New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures”, Biochemistry, 1980, 19 (11), 2396.
2) R. Y. Tsien, “A Non-disruptive Technique for Loading Calcium Buffers and Indicators into Cells”, Nature, 1981, 290, 527.
3) J. I. Korenbrot, D. L. Ochs, J. A. Williams, D. L. Miller and J. E. Brown, “The Use of Tetracarboxylate Fluorescent Indicators in the Measurement and Control of Intracellular Free Calcium Ions”, Soc. Gen. Physiol. Ser., 1986, 40, 347.
4) S. M. Harrison and D. M. Bers, “The Effect of Temperature and Ionic Strength on the Apparent Ca-affinity of EGTA and the Analogous Ca-chelators BAPTA and Dibromo-BAPTA”, Biochim. Biophys. Acta, 1987, 925, 133.
5) E. W. Gelfand and R. K. Cheung, “Dissociation of Unidirectional Influx of External Ca²⁺ and Release from Internal Stores in Activated Human T Lymphocytes”, Eur. J. Immunol., 1990, 20, 1237.
6) J. P. Kao, J. M. Alderton, R. Y. Tsien and R. A. Steinhardt, “Active Involvement of Ca²⁺ in Mitotic Progression of Swiss 3T3 Fibroblasts”, J. Cell Biol., 1990, 111, 183.
7) M. L. Schubert and J. Hightower, “Functionally Distinct Muscarinic Receptors on Gastric Somatostatin Cells”, Am. J. Physiol., 1990, 258, G982.
8) Y. Tojyo and Y. Matsumoto, “Inhibitory Effects of Loading with the Calcium-chelator 1, 2-Bis(o-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA) on Amylase Release and Cellular ATP Level in Rat Parotid Cells”, Biochem. Pharmacol., 1990, 39(11), 1775.

规格性状：

该产品为无色液体。

纯度（HPLC）：98.0％以上

处理条件

1.危险物第4类，第3石油类，危险等级Ⅲ 2.无火 3.储存方法：冷冻，避光