金畔生物试剂,miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

microRNA 逆转录产品(茎环法)

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

优异的线性关系:在宽广的模板区间内具有良好的线性关系,可检出低至pg 级RNA 模板

优越的反应体系:适合的Buffer 组分及浓度,更适用于microRNA 的逆转录

简便的引物设计:提供配套的引物设计软件,使得引物设计更加简便

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

以小鼠肝脏组织Total RNA 的10 倍稀释梯度(10 pg-1 μg) 为模板,使用miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (Vazyme #MR101) 进行逆转录,得到的cDNA 使用miRNA Universal SYBR qPCR Master Mix (Vazyme #MQ101)扩增mmu-miR-16 基因。结果显示,在宽广的模板区间内,该配套产品具有优异的线性关系。

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) 是适用于茎环法miRNA cDNA 一链合成的专用试剂盒,含基因组DNA 去除步骤,可以在42℃ 2 min 条件下快速去除基因组DNA 的污染,保证后续结果更加可靠。试剂盒基于的HiScript® II Reverse Transcriptase 具有较高的热稳定性,配以针对优化的缓冲体系,均有利于miRNA 特异性逆转录产物的生成。cDNA 产物后续定量,推荐使用本公司的miRNA Universal SYBR qPCR Master Mix (Vazyme # MQ101),以获得优异的实验结果。同时,配套推出miRNA逆转录茎环引物和定量引物的设计软件,操作简单,为miRNA 实验保驾护航。

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

-20℃保存。