–  RNA Sample Preparation
 –  Second Strand Synthesis of cDNA

The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow. 

The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module is Designed for Use with the

Following:

 –  NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771)

 –  NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)

 –  NEBNext Ultra II Ligation Module (NEB #E7595)

 –  NEBNext Ultra™ II Q5^® Master Mix (NEB #M0544)

 –  NEBNext^® Oligos for Illumina^® (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600,

    #E7535,  #E7350)

NEBNext^® Second Strand Synthesis Enzyme Mix

NEBNext^® Second Strand Synthesis Reaction Buffer