… from MACHEREY-NAGEL : NUCLEOSIL®
Classical and new generation silica manufactured by MACHEREY-NAGEL.
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Explore NUCLEOSIL® |
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| The key features of NUCLEOSIL® include: | ||||||||
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NUCLEOSIL®, a classical silica manufactured by MACHEREY-NAGEL GmbH & Co. KG is available in a wide range of pore sizes. NUCLEOSIL 100Å and 120Å have similar relative retention characteristics. NUCLEOSIL 100Å has the greater capacity whilst the 120Å material exhibits the lower column back pressure. The wide pore NUCLEOSIL 300Å silica is used as the base material for the reversed-phase bioanalytical columns.NUCLEOSIL 100-5C18AB was the first of the new generation NUCLEOSIL materials which include the HD high density phases NUCLEOSIL Protect 1 and the Nautilus, for use with up to 100% aqueous eluents. |
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Material Characteristics
| NUCLEOSIL Phase | Functional Group | Encapped | Particle Size (µm) | Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | |
|---|---|---|---|---|---|---|---|
| C2 | Dimethyl | No | 7 | 100 | 350 | 3.5 | |
| C4 | Butyl | Yes | 5 | 300 | 100 | 2 | |
| C8 | Octyl |
No No No |
5, 7, 10 3, 5, 7, 10 5 |
100 120 300 |
350 200 100 |
8.5 6.5 3 |
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| C18 | Octadecyl |
Yes Yes Yes |
3, 5, 7, 10 3, 5, 7, 10 5 |
100 120 300 |
350 200 100 |
15 11 6.5 |
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| C18AB | Octadecyl Polymeric modification | Yes | 5 |
100 |
350 | 24 | |
| C6H5 | Phenyl |
No No |
5, 7 5, 7 |
100 120 |
350 200 |
8 6.5 |
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| CN | Cyano | No |
5, 10 7 |
100 120 |
350 200 |
5 |
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| NH2 | Amino | No |
5 7 |
100 120 |
350 200 |
3.5 | |
| NO2 | Nitro | No |
5 |
100 | 350 | – | |
| OH | Diol | No | 7 | 100 | 350 | 5 | |
| SA | Sulphonic Acid | No | 5, 10 | 100 | 350 | 6.5 | |
| SB | Quaternary Ammonium | No | 5, 10 | 100 | 350 | 10 | |
| POLYGOSIL, wider pore NUCLEOSIL® (500, 1000 and 4000A) and columns for special applications eg PAH can be supplied on request. | |||||||
NUCLEOSIL® Base Deactivated Phases |
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NUCLEOSIL® 100-5 C18 AB, NUCLEOSIL® 100-5 C8/C18 HD and NUCLEOSIL® 100-5 Protect 1 represent three successive generations of base deactivated reversed phase HPLC columns, manufactured from purer silica. NUCLEOSIL® 100-5 C18 HD features a high-density mono-layer coverage. Due to the shorter chain and anincorporated group, NUCLEOSIL®100-5 Protect 1 exhibits a distinctive polar selectivity. The HD material is also offered in an octyl-bonded chemistry. Figure 1 compares the selectivity differences of four NUCLEOSIL® base deactivated phases. For the Protect 1 phase, elution of naphthalene and ethylbenzene is reversed.
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| NUCLEOSIL Phase | C8HD |
C18HD | Protect 1 | |
|---|---|---|---|---|
| Functional Group | Octyl | Octydecyl, high density surface coverage | High polarity, base deactivated | |
| Particle Size (µm) |
100 | 100 | 100 | |
| Pore Size (Å) | 3, 5 | 3, 5 | 3, 5 | |
| Carbon Load (%) | 13 | 20 | 11 | |
| Endcapped | Yes | Yes | Yes |
NUCLEOSIL® C18 Nautilus |
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NUCLEOSIL® C18 Nautilus shows outstanding stability in highly aqueous media (>95%) due to a covalently bonded C18 silane with an embedded polar functional group. As well as the hydrophobic interactions between analyte and stationary phase shown by standard C18 phases, NUCLEOSIL C18 Nautilus also shows polar interaction via hydrogen bonding and dipole-dipole forces. This can influence retention time and improve selectivity, particularly for the separation of polar compounds |
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| NUCLEOSIL® C18 Nautilus Phase | |||
|---|---|---|---|
| Pore Size (Å) |
Particle Size (µm) |
Carbon Load (%) | Endcapped |
| 100 | 3, 5 | 16 | Yes |
Contact Hichrom for further infomation
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