识别位点
特性
通过质谱进行蛋白质组学分析的理想用酶无蛋白酶污染
最适用于多肽的鉴定
概述
蛋白内切酶 LysC（flavastacin）是丝氨酸蛋白内切酶，克隆自产酶溶杆菌，能特异性切割赖氨酸羧基端的肽键。该酶是测序级酶适用于蛋白质组学和糖生物学应用。
注意：在 200 μl 双蒸水中重溶 LysC，制成 100 ng/μl 含10 mM Tris-HCl, pH 8.0 的溶液。溶液可以在 4℃ 储存几天，或者分装成一次性使用量于 -20℃ 储存几个月。
来源
提纯自产酶溶杆菌（Lysobacter enzymogenes）。
分子量
30,000 daltons。
重溶
蛋白内切酶 LysC 需溶解于 200 μl 双蒸水中制成 100 ng/μl 含10 mM Tris-HCl, pH 8.0 的溶液。快速自裂解效率随酶浓度不同而异。
| M0280S | Uracil-DNA Glycosylase （UDG） | 1,000 units |
| M0280L | Uracil-DNA Glycosylase （UDG） | 5,000 units |
| M0281S | Uracil Glycosylase Inhibitor （UGI） | 200 units |
| M0281L | 1,000 units | |
| Uracil Glycosylase Inhibitor （UGI） | ||
| M0282S | 1,000 units | |
| APE 1 | ||
| M0282L | 5,000 units | |
| APE 1 | ||
| M0284S | Multiplex PCR 5X Master Mix | 100 rxns （50 µl vol） |
| M0285S | Taq 5X Master Mix（50 µl vol） | 100 rxns |
| M0285L | Taq 5X Master Mix（50 µl vol） | 500 rxns |
| M0287S | LongAmp Taq 2X Master Mix（50 µl vol） | 100 rxns |
| M0287L | 500 rxns | |
| LongAmp Taq 2X Master Mix（50 µl vol） | ||
| M0288S | RNase HII | 250 units |
| M0288L | RNase HII | 1,250 units |
| M0289V | Antarctic Phosphatase | 500 units |
| M0289S | Antarctic Phosphatase | 1,000 units |
| M0289L | Antarctic Phosphatase | 5,000 units |
| M0290V | 500 units | |
| Alkaline Phosphatase, Calf Intestinal （CIP） | ||
| M0290S | Alkaline Phosphatase, Calf Intestinal （CIP） | 1,000 units |
| M0290L | 5,000 units | |
| Alkaline Phosphatase, Calf Intestinal （CIP） | ||
| M0291S | Tma Endonuclease III | 500 units |
| M0291L | Tma Endonuclease III | 2,500 units |
| M0293V | Exonuclease I （E. coli） | 1,500 units |
| M0293S | Exonuclease I （E. coli） | 3,000 units |
| M0293L | Exonuclease I （E. coli） | 15,000 units |
| M0294S | Tth Endonuclease IV | 500 units |
| M0294L | Tth Endonuclease IV | 2,500 units |
| M0296S | Thermostable Inorganic Pyrophosphatase | 250 units |
| M0296L | Thermostable Inorganic Pyrophosphatase | 1,250 units |
| M0297S | RNase H | 250 units |
| M0297L | RNase H | 1,250 units |
| M0298S | Cre Recombinase | 50 units |
| M0298L | Cre Recombinase | 250 units |
| M0299S | Endonuclease VIII | 1,000 units |
| M0299L | Endonuclease VIII | 5,000 units |
| M0300S | T4 Gene 32 Protein | 100 µg |
| M0300L | T4 Gene 32 Protein | 500 µg |
| M0301S | Topoisomerase I （E. coli） | 100 units |
| M0301L | Topoisomerase I （E. coli） | 500 units |
| M0302S | T7 Endonuclease I | 250 units |
| M0302L | T7 Endonuclease I | 1,250 units |
| M0303S | DNase I （RNase-free） | 1,000 units |
| M0303L | DNase I （RNase-free） | 5,000 units |
| M0304S | Endonuclease IV | 1,000 units |
| M0304L | Endonuclease IV | 5,000 units |
| M0305S | Endonuclease V | 250 units |
| M0305L | Endonuclease V | 1,250 units |
| M0306S | DNA Gyrase （E. coli） | 100 units |
| M0306L | 500 units | |
| DNA Gyrase （E. coli） | ||
| M0307S | RNase Inhibitor | 2,000 units |
| M0307L | RNase Inhibitor | 10,000 units |
| M0308S | T4 PDG （Endonuclease V） | 2,000 units |
| M0308L | T4 PDG （Endonuclease V） | 10,000 units |
| M0309S | PreCR Repair Mix | 30 rxns |
| M0309L | PreCR Repair Mix | 150 rxns |
| M0310S | p19 siRNA Binding Protein | 1,000 units |
| M0310L | p19 siRNA Binding Protein | 5,000 units |
| M0313S | Human Alkyladenine Glycosylase （hAAG） | 500 units |
| M0313L | Human Alkyladenine Glycosylase （hAAG） | 2,500 units |
| M0314S | Murine RNase Inhibitor | 3,000 units |
| M0314L | Murine RNase Inhibitor | 15,000 units |
| M0315S | Terminal Transferase | 500 units |
| M0315L | Terminal Transferase | 2,500 units |
| M0320V | Taq DNA Polymerase with Standard Taq （Mg-free） Buffer | 200 units |
| M0320S | Taq DNA Polymerase with Standard Taq （Mg-free） Buffer | 400 units |
| M0320L | Taq DNA Polymerase with Standard Taq （Mg-free） Buffer | 2,000 units |
| M0321V | Taq DNA Polymerase with ThermoPol II （Mg-free） Buffer | 200 units |
| M0321S | Taq DNA Polymerase with ThermoPol II （Mg-free） Buffer | 400 units |
| M0321L | Taq DNA Polymerase with ThermoPol II （Mg-free） Buffer | 2,000 units |
| M0323V | LongAmp Taq DNA Polymerase | 250 units |
| M0323S | LongAmp Taq DNA Polymerase | 500 units |
| M0323L | LongAmp Taq DNA Polymerase | 2,500 units |
| M0324V | Crimson Taq DNA Polymerase | 100 units |
| M0324S | Crimson Taq DNA Polymerase | 200 units |
| M0324L | Crimson Taq DNA Polymerase | 1,000 units |
| M0325V | Crimson Taq DNA Polymerase with （Mg-free） Buffer | 100 units |
| M0325S | Crimson Taq DNA Polymerase with （Mg-free） Buffer | 200 units |
| M0325L | Crimson Taq DNA Polymerase with （Mg-free） Buffer | 1,000 units |
| M0326V | Crimson LongAmp Taq DNA Polymerase | 125 units |
| M0326S | Crimson LongAmp Taq DNA Polymerase | 250 units |
| M0326L | Crimson LongAmp Taq DNA Polymerase | 1,250 units |
| M0327V | Sulfolobus DNA Polymerase IV | 50 units |
| M0327S | Sulfolobus DNA Polymerase IV | 100 units |
| M0327L | Sulfolobus DNA Polymerase IV | 500 units |
| M0328V | Bst DNA Polymerase, Full Length | 250 units |
| M0328S | Bst DNA Polymerase, Full Length | 500 units |
| M0328L | Bst DNA Polymerase, Full Length | 2,500 units |
| M0330V | Bsu DNA Polymerase, Large Fragment | 100 units |
| M0330S | Bsu DNA Polymerase, Large Fragment | 200 units |
| M0330L | Bsu DNA Polymerase, Large Fragment | 1,000 units |
| M0331S | 5´ Deadenylase | 1,000 units |
| M0332V | Hemo KlenTaq | 100 rxns （25 µl vol） |
| M0332S | Hemo KlenTaq | 200 rxns （25 µl vol） |
| M0332L | Hemo KlenTaq | 1,000 rxns （25 µl vol） |
| M0333V | Therminator III DNA Polymerase | 100 units |
| M0333S | Therminator III DNA Polymerase | 200 units |
| M0333L | Therminator III DNA Polymerase | 1,000 units |
| M0334S | Therminator γ DNA Polymerase | 200 units |
| M0334L | Therminator γ DNA Polymerase | 1,000 units |
| M0335S | hNEIL1 | 500 units |
| M0335L | hNEIL1 | 2,500 units |
| M0336S | hSMUG1 | 500 units |
| M0336L | hSMUG1 | 2,500 units |
| M0337S | Poly（U） Polymerase | 60 units |
| M0338S | XRN-1 | 20 units |
| M0392S | b-Agarase I | 100 units |
| M0392L | b-Agarase I | 500 units |
| M0393S | Apyrase | 10,000 milliunits |
| M0393L | Apyrase | 50,000 milliunits |
| M0394S | ATP Sulfurylase | 10 units |
| M0394L | 50 units | |
| ATP Sulfurylase | ||
| M2200V | Quick Ligation Kit | 15 rxns |
| M2200S | Quick Ligation Kit | 30 rxns |
| M2200L | Quick Ligation Kit | 150 rxns |
| M2401S | ET SSB （Extreme Thermostable ssBinding Protein） | 50 µg |
| M2402S | Tth RecA | 100 µg |
| M2403S | Pyrophosphatase, inorganic （yeast） | 10 units |
| M2403L | Pyrophosphatase, inorganic （yeast） | 50 units |
| M2501S | Histone H1 Human, Recombinant | 100 µg |
| M2502S | Histone H2A Human, Recombinant | 100 µg |
| M2503S | Histone H3.1 Human, Recombinant | 100 µg |
| M2504S | Histone H4 Human, Recombinant | 100 µg |
| M2505S | Histone H2B Human, Recombinant | 100 µg |
| M2506S | Histone H3.2 Human, Recombinant | 100 µg |
| M2507S | Histone H3.3 Human, Recombinant | 100 µg |
| M2551S | TransPass R1 Transfection Reagent | 0.4 ml |
| M2552S | TransPass R2 Transfection Reagent | 1.0 ml |
| M2553S | 0.6 ml | |
| TransPass D1 Transfection Reagent | ||
| M2553L | TransPass D1 Transfection Reagent | 3.0 ml |
| M2554S | TransPass D2 Transfection Reagent | 0.6 ml |
| M2554L | TransPass D2 Transfection Reagent | 3.0 ml |
| M2556S | TransPass HeLa Transfection Reagent | 2 x 0.4 ml |
| M2557S | 1.2 ml | |
| TransPass COS/293 Transfection Reagent | ||
| M2558S | TransPass HUVEC Transfection Reagent | 1.8 ml |
| M2561S | TransPass V | 0.6 ml |
| M2563S | TransPass P Protein Transfection Reagent | 0.125 ml |
| M5505S | USER Enzyme | 50 units |
| M5505L | USER Enzyme | 250 units |
| N0315S | M13K07 Helper Phage | 1.8 ml |
| N0316S | M13KE Phage | 0.04 ml |
| N0340S | Lambda Ladder PFG Marker | 50 gel lanes |
| N0345S | Yeast Chromosome PFG Marker | 50 gel lanes |
| N0350S | Low Range PFG Marker | 50 gel lanes |
| N0356S | dm5CTP | 1 µmol |
| N0362S | ssRNA Ladder | 25 gel lanes |
| N0363S | dsRNA Ladder | 25 gel lanes |
| N0364S | Low Range ssRNA Ladder | 25 gel lanes |
| N0417S | pLox2+ （linearized） | 10 µg |
| N0445S | 7-deaza-dGTP | 0.15 µmol |
| N0446S | Deoxynucleotide Solution Set | 25 µmol of each |
| N0447V | Deoxynucleotide Solution Mix | 4 µmol of each |
| N0447S | Deoxynucleotide Solution Mix | 8 µmol of each |
| N0447L | Deoxynucleotide Solution Mix | 40 µmol of each |
| N0460S | Acyclonucleotide Set | 0.5 µmol of each |
| N0466S | Ribonucleotide Solution Mix | 8 µmol of each |
| N0466L | Ribonucleotide Solution Mix | 40 µmol of each |
| N0467V | Quick-Load 100 bp DNA Ladder | 37.5 gel lanes |
| N0467S | Quick-Load 100 bp DNA Ladder | 125 gel lanes |
| N0467L | Quick-Load 100 bp DNA Ladder | 375 gel lanes |
| N0468V | Quick-Load 1 kb DNA Ladder | 37.5 gel lanes |
| N0468S | Quick-Load 1 kb DNA Ladder | 125 gel lanes |
| N0468L | Quick-Load 1 kb DNA Ladder | 375 gel lanes |
| N0469V | Quick-Load 2-log DNA Ladder （0.1-10.0 kb） | 37.5 gel lanes |
| N0469S | Quick-Load 2-log DNA Ladder （0.1-10.0 kb） | 125 gel lanes |
| N0469L | Quick-Load 2-log DNA Ladder （0.1-10.0 kb） | 375 gel lanes |
| N0471S | DNA Gyrase （E. coli） Substrate | 20 µg |
| N0472S | Supercoiled DNA Ladder | 100 gel lanes |
| N1500S | NEBlot Kit | 25 rxns |
| N1500L | NEBlot Kit | 50 rxns |
| N1501S | Octadeoxyribonucleotides in 10X Labeling Buffer | 25 rxns |
| N1501L | Octadeoxyribonucleotides in 10X Labeling Buffer | 50 rxns |
| N2100S | Fluorescein-siRNA Transfection Control | 1 nmol |
| N2101S | siRNA Marker | 100 gel lanes |
| N2102S | microRNA Marker | 100 gel lanes |
| N3011S | Lambda DNA | 250 µg |
| N3011L | Lambda DNA | 1,250 µg |
| N3012S | Lambda DNA-HindIII Digest | 150 gel lanes |
| N3012L | Lambda DNA-HindIII Digest | 750 gel lanes |
| N3013S | Lambda DNA （N6-methyladenine-free） | 250 µg |
| N3013L | Lambda DNA （N6-methyladenine-free） | 1,250 µg |
| N3014S | Lambda DNA-BstEII Digest | 150 gel lanes |
| N3014L | Lambda DNA-BstEII Digest | 750 gel lanes |
| N3019S | Lambda DNA-Mono Cut Mix | 100 gel lanes |
| N3019L | Lambda DNA-Mono Cut Mix | 500 gel lanes |
| N3021S | PhiX174 RF I DNA | 30 µg |
| N3021L | PhiX174 RF I DNA | 150 µg |
| N3022S | PhiX174 RF II DNA | 30 µg |
| N3022L | PhiX174 RF II DNA | 150 µg |
| N3023S | PhiX174 Virion DNA | 50 µg |
| N3023L | PhiX174 Virion DNA | 250 µg |
| N3026S | PhiX174 DNA-HaeIII Digest | 50 gel lanes |
| N3026L | PhiX174 DNA-HaeIII Digest | 250 gel lanes |
| N3031S | pBR322 DNA-BstNI Digest | 50 gel lanes |
| N3031L | pBR322 DNA-BstNI Digest | 250 gel lanes |
| N3032S | pBR322 DNA-MspI Digest | 50 gel lanes |
| N3032L | pBR322 DNA-MspI Digest | 250 gel lanes |
| N3033S | pBR322 Vector | 50 µg |
| N3033L | pBR322 Vector | 250 µg |
| N3034S | pKLAC1-GLuc Control Plasmid | 20 µg |
| N3041S | pUC19 Vector | 50 µg |
| N3041L | pUC19 Vector | 250 µg
|
