ProSpec,CD33 Human Sf9,CD33 人重组体Sf9 PRO-2492

在免疫反应中，CD33 作为配体诱导的酪氨酸磷酸化的抑制性受体，通过其 SH2 结构域募集细胞质磷酸酶，通过信号分子的去磷酸化阻断信号转导。ProSpec,CD33 Human Sf9,CD33 人重组体Sf9 PRO-2492

  • CD33 Human, Sf9

  • CD33 Human Recombinant, Sf9
  • PRO-2492

Catalogue number

PRO-2492

Synonyms

Myeloid cell surface antigen CD33 isoform 1, CD33, FLJ00391, p67, SIGLEC-3, SIGLEC3, gp67.

Introduction

CD33 is putative adhesion molecule of myelomonocytic-derived cells which mediates sialic-acid dependent binding to cells. CD33 prefers to bind to alpha-2,6-linked sialic acid. The sialic acid recognition site is masked by cis interactions with sialic acids on the same cell surface. In the immune response, CD33 perform as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatases through their SH2 domains which block signal transduction through dephosphorylation of signaling molecules. In addition, CD33 induces apoptosis in acute myeloid leukemia.

CD33 是骨髓单核细胞衍生细胞的推定粘附分子，它介导唾液酸依赖性与细胞的结合。 CD33 更喜欢与 α-2,6 连接的唾液酸结合。 唾液酸识别位点被同一细胞表面上与唾液酸的顺式相互作用所掩盖。 在免疫反应中，CD33 作为配体诱导的酪氨酸磷酸化的抑制性受体，通过其 SH2 结构域募集细胞质磷酸酶，通过信号分子的去磷酸化阻断信号转导。 此外，CD33 在急性髓性白血病中诱导细胞凋亡。

Description

CD33 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain and fused to a 239 aa hIgG-His Tag at C-terminus containing a total of 484 amino acids and having a molecular mass of 54kDa. CD33 shows multiple bands between 50-70kDa on -PAGE, reducing conditions and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

CD33 protein solution contains Phosphate buffered saline & 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

ADLDPNFWLQ VQESVTVQEG LCVLVPCTFF HPIPYYDKNS PVHGYWFREG AIISGDSPVA TNKLDQEVQE ETQGRFRLLG DPSRNNCSLS IVDARRRDNG SYFFRMERGS TKYSYKSPQL SVHVTDLTHR PKILIPGTLE PGHSKNLTCS VSWACEQGTP PIFSWLSAAP TSLGPRTTHS SVLIITPRPQ DHGTNLTCQV KFAGAGVTTE RTIQLNVTYV PQNPTTGIFP GDGSGKQETR AGVVHLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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