ProSpec-C-10 Mouse/CHM-307,C-10 小鼠重组/CHM-307

Small inducible cytokine A6, CCL6, C10 protein, c10, MRP-1, Scya6, chemokine ligand 6.

Chemokine ligand 6 is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.
In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

趋化因子配体 6 是一种小细胞因子，属于 CC 趋化因子家族，仅在啮齿动物中发现。
在小鼠中，CCL6 在来自嗜中性粒细胞和巨噬细胞谱系的细胞中表达，并且可以在适合骨髓细胞分化的条件下被大量诱导。 它在用细胞因子 GM-CSF 刺激的骨髓培养物中高度表达。 某些低水平的基因表达也出现在某些骨髓来源的细胞系中，这些细胞系也可以在 GM-CSF 培养中被大大诱导。 然而，在活化的 T 细胞系中，CCL6 的表达大大降低。 CCL6 也可以在小鼠肺中由细胞因子白介素 13 诱导。小鼠 CCL6 位于 11 号染色体上。CCL6 的细胞表面受体被认为是趋化因子受体 CCR1。

C-10 Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 95 amino acids and having a molecular mass of 10.7kDa.
The CCL6 is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

CCL6 Mouse was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

It is recommended to reconstitute the lyophilized C10 protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized C10 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA.

The Biological activity was determined by its ability to chemoattract human CCR1 transfected BaF3 mouse proB cells using a concentration range of 0.05-0.25µg/ml.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.