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IL 8 Porcine
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Interleukin-8 Porcine Recombinant
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CHM-340
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Catalogue number
CHM-340
Synonyms
IL-8, CXCL8, Monocyte-derived neutrophil chemotactic factor, MDNCF, T-cell chemotactic factor, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil-activating peptide, MONAP, Emoctakin, K60, NAF, LECT, LUCT, 3-10C, LYNAP, SCYB8, TSG-1, AMCF-I, b-ENAP, Alveolar macrophage chemotactic factor I.
Introduction
Interleukin-8 is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor.
白细胞介素 8 是一种由巨噬细胞和其他细胞类型(如上皮细胞)产生的趋化因子。 它也由内皮细胞合成,内皮细胞将 IL-8 储存在它们的储存囊泡 Weibel-Palade 体中。 当第一次遇到抗原时,遇到它的原代细胞是吞噬颗粒的巨噬细胞。 在处理后,它们会释放趋化因子以向其他免疫细胞发出信号,使其进入炎症部位。 IL-8 就是这样一种趋化因子。 它作为一种化学信号,在炎症部位吸引中性粒细胞,因此也称为中性粒细胞趋化因子。
Description
Interleukin-8 Porcine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 78 amino acids and having a molecular mass of 9.1kDa.
The IL8 is purified by proprietary chromatographic techniques.
The IL8 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized Interleukin 8 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Interleukin-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
Please prevent freeze-thaw cycles.
冻干的白细胞介素 8 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 复溶后,CXCL8 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。
请防止冻融循环。
Purity
Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.
Analysis by RP-HPLC.
Analysis by -PAGE.
Biological Activity
The biological activity was determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml.
Amino acid sequence
ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ.
Usage
Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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