ProSpec,IP 10 Mouse,IP-10 小鼠重组CHM-336

Small inducible cytokine B10, CXCL10, 10 kDa, Gamma-IP10, IP-10, chemokine ligand 10, C7, IFI10, INP10, crg-2, mob-1, SCYB10, gIP-10.

Chemokine ligand 10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes and T cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. The three-dimensional crystal structure of this chemokine has been determined under 3 different conditions to a resolution of up to 1.92A.

趋化因子配体 10 是属于 CXC 趋化因子家族的小细胞因子。 CXCL10 由几种细胞类型分泌。 这些细胞类型包括单核细胞、内皮细胞和成纤维细胞。 CXCL10 具有多种作用，例如对单核细胞和 T 细胞的化学吸引、促进 T 细胞与内皮细胞的粘附、抗肿瘤活性以及抑制骨髓集落形成和血管生成。 CXCL10 基因位于人类第 4 号染色体上，与其他几种 CXC 趋化因子形成一个簇。 这种趋化因子通过与细胞表面趋化因子受体 CXCR3 结合来引发其作用。 该趋化因子的三维晶体结构已在 3 种不同条件下确定，分辨率高达 1.92A。

IP-10 Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8701 Dalton.
The IP-10 is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

IP-10 was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

It is recommended to reconstitute the lyophilized IP-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized IP-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 IP-10 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，CXCL10 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP.

Determined by its ability to chemoattract hCXCR3/HEK293 cells using a concentration range of 100.0-500.0 ng/ml.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.02 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard solution of IP-10 as a Reference Standard.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.