ProSpec,MCP 1 Rat,单核细胞趋化蛋白-1大鼠重组CHM-315

Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11, Immediate-early serum-responsive JE protein.

Chemokine ligand 2 is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 . It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B . CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weightof approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.

趋化因子配体 2 是属于 CC 趋化因子家族的小细胞因子，也称为单核细胞趋化蛋白 1。 它存在于牙齿萌出和骨骼退化的部位。 在骨骼中，CCL2 由成熟的破骨细胞和成骨细胞表达，并受核因子 βB 的控制。 CCL2 将免疫细胞（例如单核细胞）募集到组织损伤和感染部位。 这种趋化因子作为蛋白质前体产生，含有 23 个氨基酸的信号肽和 76 个氨基酸的成熟肽。 它是一种单体多肽，分子量约为13kDa。 与许多其他 CC 趋化因子一样，CCL2 位于人类的第 17 号染色体上。 结合 CCL2 的细胞表面受体是 CCR2 和 CCR5。

Monocyte Chemotactic Protein-1 Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 125 amino acids and having a molecular mass of 14.1 kDa.
The MCP-1 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的单核细胞趋化蛋白 1 大鼠重组体是一种非糖基化的多肽链，含有 125 个氨基酸，分子量为 14.1 kDa。
MCP-1 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The protein was lyophilized from a concentrated sterile solution containing no additives.

It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 98.0% as determined by -PAGE.

QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN.

ED50 =1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay.

Title: Adiponectin Reduces Hepatic Stellate Cell Migration by Promoting Tissue Inhibitor of Metalloproteinase-1 Secretion
Publications: Journal of Biological Chemistry : jbc-M114.
Link: http://www.jbc.org/content/early/2015/01/09/jbc.M114.598011.full.pdf

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.