ProSpec CHM-250,MDC Human,巨噬细胞衍生的趋化因子人重组CHM-250

C-C motif chemokine 22, Small-inducible cytokine A22, Macrophage-derived chemokine, MDC, Stimulated T-cell chemotactic protein 1, CC chemokine STCP-1, CCL22, MDC, SCYA22, ABCD-1, DC/B-CK, MGC34554, A-152E5.1.

MDC is a small cytokine that belongs to the CC chemokine family. CCL22 is one of several Cys-Cys cytokine genes clustered on the q arm of chromosome 16.
MDC shows chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. On the other hand, MDC shows a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. MDC may also have a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. MDC interacts with cell surface chemokine receptors CCR4.
CCL22 is vastly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. CCL22 is also found in the lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression of CCL22 can be seen in the lung and the spleen and very weak expression in the small intestine. In the lymph node CCL22 is expressed in a mature subset of Langerhans’ cells .
Furthermore, CCL22 is expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. In addition, MDC has a role in hindering progression of lung cancer. Moreover, significantly higher CCL22 expression is linked to gastric cancer.

MDC是属于CC趋化因子家族的小细胞因子。 CCL22 是聚集在 16 号染色体 q 臂上的几个 Cys-Cys 细胞因子基因之一。
MDC 对自然杀伤细胞、慢性活化的 T 淋巴细胞、单核细胞和树突状细胞具有趋化活性。另一方面，MDC 对原代活化的 T 淋巴细胞表现出温和的活性，对中性粒细胞、嗜酸性粒细胞和静息 T 淋巴细胞没有趋化活性。 MDC 还可能在活化 T 淋巴细胞向炎症部位的运输和活化 T 淋巴细胞生理学的其他方面发挥作用。 MDC 与细胞表面趋化因子受体 CCR4 相互作用。
CCL22 在巨噬细胞和单核细胞衍生的树突细胞和胸腺中大量表达。 CCL22 也存在于淋巴结、阑尾、活化的单核细胞、静息和活化的巨噬细胞中。 CCL22在肺和脾中的表达较低，在小肠中表达非常弱。在淋巴结中，CCL22 在成熟的朗格汉斯细胞亚群中表达。
此外，CCL22 在表皮和真皮的特应性皮炎、过敏性接触性皮炎皮肤和牛皮癣中均有表达。此外，MDC 具有阻碍肺癌进展的作用。此外，显着更高的 CCL22 表达与胃癌有关。

MDC Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 69 amino acids and having a molecular mass of 8.1 kDa.
The MDC is purified by proprietary chromatographic techniques.

Escherichia Coli.

Filtered White lyophilized powder.

CCL22 filtered and lyophilized from a concentrated solution containing 20mM phosphate buffer & 500mM NaCl pH-7.4.

It is recommended to reconstitute the lyophilized CCL22 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Lyophilized CCL22 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL22 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Determined by its ability to chemoattract human T cells using a concentration range of 10ng-100ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

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Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.