ProSpec,CCL28 Human,黏膜相关上皮趋化因子人重组CHM-353

MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.

CCL28 is part of the subfamily of small cytokine CC genes. CCL28 shows chemotactic activity for resting CD4 or CD8 T cells and eosinophils. CCL28 binds to chemokine receptors CCR3 and CCR10. CCL28 is involved in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. CCL28 mediates mucosal immunity in HIV exposure and infection. CCL28 is involved in the pathogenesis of inflammatory skin diseases.
Human CCL28 cDNA encodes a 127 amino acid residue precursor protein with a putative 22 amino acid residue signal peptide that is cleaved to produce the 105 amino acid residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% amino acid identity in their mature regions. CCL28 shares the most homology with CCL27/CTACK. Human and mouse CCL28 RNA expression was found to be highest in normal and pathologic colon with the protein being expressed by epithelial cells. Human CCL28 RNA was also present in normal and asthmatic lung tissues.

CCL28 是小细胞因子 CC 基因亚家族的一部分。 CCL28 显示对静息 CD4 或 CD8 T 细胞和嗜酸性粒细胞的趋化活性。 CCL28 与趋化因子受体 CCR3 和 CCR10 结合。 CCL28 参与皮外上皮组织的生理学，包括多种黏膜器官。 CCL28 在 HIV 暴露和感染中介导黏膜免疫。 CCL28 参与炎症性皮肤病的发病机制。
人 CCL28 cDNA 编码一个 127 个氨基酸残基的前体蛋白和一个推定的 22 个氨基酸残基信号肽，该信号肽被切割以产生 105 个氨基酸残基的成熟蛋白。 人和小鼠 CCL28 高度保守，在其成熟区域共享 83% 的氨基酸同一性。 CCL28 与 CCL27/CTACK 的同源性最高。 发现人和小鼠 CCL28 RNA 表达在正常和病理结肠中最高，该蛋白质由上皮细胞表达。 人 CCL28 RNA 也存在于正常和哮喘肺组织中。

CCL28 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 108 amino acids and having a molecular mass of 12.3 kDa.
The CCL28 is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a concentrated solution in water containing 20mM PBS and 150mM NaCl.

It is recommended to reconstitute the lyophilized CCL28 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 CCL28，然后可以进一步稀释成其他水溶液。

Lyophilized CCL28 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL28 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY.

Determined by its ability to chemoattract human lymphocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.