ProSpec,MIP 1b Human CHM-276,巨噬细胞炎症蛋白-1 Beta 人重组CHM-276

Small inducible cytokine A4, CCL4, Macrophage inflammatory protein 1-beta, MIP-1- beta, MIP-1-beta, T-cell activation protein 2, ACT-2, PAT 744, H400, SIS-gamma, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, chemokine ligand 4, ACT2, G-26, LAG1, MIP1B, SCYA4, AT744.1, MGC104418, MGC126025, MGC126026.

Macrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now also named CCL3 and CCL4. Both factors are produced by macrophages after they are stimulated with bacterial endotoxins. MIP-1a and MIP-1b activate human granulocytes which can lead to acute neutrophilic inflammation. MIP-1a and MIP-1b induce synthesis and release of other pro-inflammatory cytokines such as interleukin-1 , IL-6 and TNF-alpha from fibroblasts and macrophages. CCL3 and CCL4 genes are both located on human chromosome 17.

巨噬细胞炎症蛋白属于称为趋化因子的趋化细胞因子家族。 在人类中，有两种主要形式，即 MIP-1a 和 MIP-1b，现在也被命名为 CCL3 和 CCL4。 这两种因子都是巨噬细胞在受到细菌内毒素刺激后产生的。 MIP-1a 和 MIP-1b 激活可导致急性中性粒细胞炎症的人粒细胞。 MIP-1a和MIP-1b诱导成纤维细胞和巨噬细胞合成和释放其他促炎细胞因子，例如白细胞介素1、IL-6和TNF-α。 CCL3 和 CCL4 基因都位于人类第 17 号染色体上。

Macrophage Inflammatory Protein-1 beta Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7620 Dalton.
The CCL4 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的巨噬细胞炎症蛋白-1 β 人重组体是一种单一的、非糖基化的多肽链，含有 69 个氨基酸，分子量为 7620 道尔顿。
CCL4 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a concentrated solution in water containing no additives.

It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 99.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Met-Gly-Ser.

The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000IU/mg.

Title: CONFUSION BETWEEN CCL4 AND CCL4L1: AN EXAMPLE TO
BEAR IN MIND WHEN USING THIRD-PARTY REAGENTS.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.