ProSpec,MIP 3a Human,巨噬细胞炎症蛋白 3 α 人重组CHM-351

S Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation- regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20.

CCL-20 is a chemotactic factor that draws lymphocytes & neutrophils, rathar than monocytes. MIP-3 alpha inhibits proliferation of myeloid progenitors in colony formation assays. MIP3A plays a role in the formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. CCL-20 holdes antibacterial activity e.coli atcc 25922 and s.aureus atcc 29213. CCL-20 gene transcription is activated by H. pylori, which activates NF-kappaB through intracellular signal pathway which involves IkappaB kinase and NF-kappaB-inducing kinase. MIP-3 alpha is invloved in chemokine-mediated lymphocyte trafficking during gastric inflammation in Helicobacter infection. CCL-20 expression is involved in the recruitment of CD45R0-positive T cell subsets into the intestinal lamina propria.
MIP-3A is in charge of the advancement of pulpal inflammation through the recruitment of C-C motif Receptor 6-expressing lymphocytes Vaginal epithelial cells respond to factors present in semen by secreting MIP-3 alpha, which increases langerhans cells recruitment during HIV transmission.

CCL-20 是一种趋化因子，它比单核细胞更能吸引淋巴细胞和中性粒细胞。 MIP-3 α 在集落形成试验中抑制骨髓祖细胞的增殖。 MIP3A 通过将淋巴细胞和树突状细胞吸引到上皮细胞，在黏膜淋巴组织的形成和功能中发挥作用。 C-末端加工形式已被证明对白细胞具有同样的趋化活性。 CCL-20 具有抗菌活性 e.coli atcc 25922 和 s.aureus atcc 29213。CCL-20 基因转录由幽门螺杆菌激活，幽门螺杆菌通过涉及 IkappaB 激酶和 NF-kappaB 诱导激酶的细胞内信号通路激活 NF-kappaB . MIP-3 α 在幽门螺杆菌感染的胃炎症期间参与趋化因子介导的淋巴细胞运输。 CCL-20 表达参与将 CD45R0 阳性 T 细胞亚群募集到肠固有层中。
MIP-3A 负责通过募集 C-C 基序受体 6 表达淋巴细胞来促进牙髓炎症阴道上皮细胞通过分泌 MIP-3 α 对精液中存在的因子作出反应，这增加了 HIV 传播过程中朗格汉斯细胞的募集。

MIP-3 Alpha Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 8 kDa.
The MIP-3a is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

It is recommended to reconstitute the lyophilized MIP-3 Alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MIP-3a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL20 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM.

Determined by its ability to chemoattract human T lymphocytes using a concentration range of 10.0 -50.0 ng/ml, corresponding to a specific activity of 20,000-100,000units/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.