ProSpec,TARC Human,胸腺和活化调节的趋化因子人重组CHM-240

C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, CCL17, CCL-17, SCYA17, TARC, A-152E5.3, MGC138271, MGC138273.

TARC cDNA encodes a 94 amino acid precursor protein with a 23 amino acid residue signal peptide that is cleaved off to generate the 71 amino acid residue mature secreted protein. Along with CC chemokine family members, CCL-17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1a, MIP-1b, MCP-1, MCP-2, MCP-3 and I-309. TARC is expressed in thymus, and at a lower level in the lung, colon, and small intestine. TARC is in addition transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant TARC has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL-17 was recently identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. CCL17 is one of quite a few Cys-Cys cytokine genes clustered on the q arm of chromosome 16. CCL17 shows chemotactic activity for T lymphocytes, but not monocytes or granulocytes. CCL17 binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.

TARC cDNA 编码一个 94 个氨基酸的前体蛋白和一个 23 个氨基酸残基的信号肽，该信号肽被切割掉以产生 71 个氨基酸残基的成熟分泌蛋白。与 CC 趋化因子家族成员一起，CCL-17 与 RANTES、MIP-1a、MIP-1b、MCP-1、MCP-2、MCP-3 和 I-309 具有大约 24-29% 的氨基酸序列同一性。 TARC 在胸腺中表达，在肺、结肠和小肠中表达水平较低。此外，TARC 在受刺激的外周血单核细胞中瞬时表达。重组 TARC 已被证明对 T 细胞系具有趋化性，但对单核细胞或中性粒细胞没有。 CCL-17 最近被确定为 CCR4 的特异性功能配体，CCR4 是一种选择性表达于 T 细胞上的受体。 CCL17 是聚集在 16 号染色体 q 臂上的大量 Cys-Cys 细胞因子基因之一。CCL17 对 T 淋巴细胞显示趋化活性，但对单核细胞或粒细胞没有趋化活性。 CCL17 与趋化因子受体 CCR4 和 CCR8 结合。这种趋化因子在胸腺 T 细胞发育以及成熟 T 细胞的运输和激活中起重要作用。

CCL17 Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 8 kDa.
The TARC is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The protein was lyophilized from a concentrated solution containing 20mM PBS & 150mM NaCl pH-7.4.

It is recommended to reconstitute the lyophilized CCL17 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized TARC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TARC should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 1.0-10.0 ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

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