- Products
- DNA
- CPG solid supports
- Universal CPG type I, 500A
Universal CPG type I, 500A
| Cat. # | Quantity | Price | Lead time | |
|---|---|---|---|---|
| 2646-1g | 1 g | 39.00$
|
||
| 2646-10g | 10 g | 351.00$
|
The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.
The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 8 hours at 80 °C using ammonium hydroxide or 17 hours at 55 °C; 3 hours at 80 °C using AMA mixture, ammonium hydroxide – 40% methylamine (1:1) or 8 hours at 55 °C using 40% methylamine or 30 minutes at 80 °C; 3 hours at 55 °C; 17 hours at room temperature using 0.4M NaOH in methanol – water (4:1).
Customers also purchased with this product
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−95 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | AMA mixture, ammonium hydroxide – 40% methylamine (1:1), 3 hours at 80 °C |
| Deprotection conditions: | identical to protected nucleobases |
