- Description
Catalogue number
CYT-1054
Synonyms
AI790464, Pro-epidermal growth factor, URG.
Introduction
Pro-Epidermal Growth Factor Isoform 1 or EGF, is a globular peptide which includes three intra molecular disulfide bonds. This protein acts as a growth factor that mediates the growth and proliferation of different epithelial & epidermal cells. Among other processes that EGF is part of are inhibition of gastric secretion and wound healing. EGF is a ligand for class I tyrosine kinase receptor .
Description
EGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8.6kDa.EGF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Physical Appearance
Formulation
EGF protein solution contains 10% glycerol, 20mM Tris-HCl , 0.1M NaCl & 2mM DTT.
Stability
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
Purity
Biological Activity
Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 for this effect less or equal to 1ng/ml.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR.
Usage
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