ProSpec-IGFBP 5 Human

IGFBP-5, IBP-5, IGF-binding protein 5.

IGFBP5 is a member of the insulin-like growth factor binding protein family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit and either insulin-like growth factor I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

IGFBP5 Human Recombinant produced in E.Coli is non-glycosylated homodimer containing 2×252 amino acids and having a molecular mass of 28.6kDa.
IGFBP5 is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

IBP-5 was lyophilized from a concentrated solution containing 10mM sodium Citrate pH-3.

It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP 5 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 96.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE.

The ED₅₀, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is < 0.4µg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15ng/ml of rHuIGF-II.

Title:Effects of Dietary Carbohydrate Modification
in Persons with the Metabolic Syndrome
– A Transcriptomics Approach in Adipose Tissue
Publication:Department of Clinical Nutrition, Food and Health Research Centre
Institute of Public Health and Clinical Nutrition
Faculty of Health Sciences
University of Eastern Finland Kuopio 2010
Link:http://epublications.uef.fi/pub/urn_isbn_978-952-61-0024-1/urn_isbn_978-952-61-0024-1.pdf

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.