ProSpec-Leptin Receptor Chicken

OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor.

Leptin Receptor is a part of the gp130 family of cytokine receptors that stimulate gene transcription by activating cytosolic STAT proteins. Leptin Receptor plays a role in the regulation of fat metabolism and in novel hematopoietic pathway that is obligatory for normal lymphopoiesis. Leptin Receptorparticipates in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus.Leptin Receptoraffectsspecifically on T lymphocyte responses.

Leptin Binding Domain Chicken Recombinant also called Leptin Receptor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Chicken Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 420-626 of chicken leptin receptor.
The Leptin Binding Domain is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered colorless solution at a concentration of 0.4 mg/ml.

The protein was filter sterilized and stored at 4°C solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl.

Sterile solutions at 0.5mg/ml or less are stable at 4°C for several months.

Greater than 99.0% as determined by:
Analysis by SEC-HPLC.
Analysis by -PAGE.

The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile Biological ActivityBiological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.

Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .

2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.