Description

Catalogue number

CYT-810

Synonyms

Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA.

Introduction

The Melanoma Inhibitory protein was identified as an inhibitor of in vitro growth of malignant melanoma cells. The protein contains a SH3 domain.

MIA acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. In a study of human melanoma cell lines with different metastatic capacity MIA mRNA expression appeared to be inversely correlated with pigmentation. MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas.

Description

MIA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 128 amino acids and having a molecular mass of 14.4 kDa.

MIA is fused to a 23 amino acid His-tag at N-terminus.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The MIA solution contains 20mM Tris-HCl buffer , 0.4M Urea and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-MIA Human, His

MIA Human, His