ProSpec-PDGF BB Human

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide.

PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor alpha polypeptide , where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.

Platelet-Derived Growth Factor BB Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2×109 amino acids and having a molecular mass of 24.3 kDa. PDGF-BB is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.

Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells.
The expected ED₅₀ for this effect is 1.0-3.0 ng/ml.

1.Title:Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway.
Publication:Published online before print October 30, 2009, doi: 10.1182/blood-2009-06-223719 Blood January 7, 2010 vol. 115 no. 1 51-60 .
Link:http://bloodjournal.hematologylibrary.org/content/115/1/51.full

2.Title: attenuates skeletal muscle dystrophy in mdx mice.
 Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548.
Link:http://www.fasebj.org/content/23/8/2539.full

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.