ProSpec-Prolactin Ovine

Mammotropin, Luteotropic hormone, Luteotropin, PRL.

Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.

Prolactin Ovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa. The Prolactin n is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The protein was lyophilized from a concentrated solution with 0.02-0.03% NaHCO₃.

It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H₂O or 0.4% NaHCO₃ adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 99.0% as determined by:
 Gel filtration analysis.
Analysis by -PAGE.

The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.

Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.

Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.93 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC DNAman computer analysis program of protein sequences.  

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

1.Title:Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.
Publication:Published online before print December 18, 2008, doi: 10.1210/en.2008-1318 Endocrinology April 1, 2009 vol. 150 no. 4 1646-1653 .
Link:http://endo.endojournals.org/content/150/4/1646.full

2.Title:ISLET ENDOTHELIAL CELLS AND PANCREATIC ?-CELL PROLIFERATION: STUDIES IN VITRO AND DURING PREGNANCY IN ADULT RATS.
Publication:Endocrinology. First published January 26, 2006 as doi:10.1210/en.2005-0997.
Link:http://endo.endojournals.org/content/early/2006/01/26/en.2005-0997.full.pdf

3.Title:Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.
Publication:Endocrinology. First published ahead of print December 18, 2008 as doi:10.1210/en.2008-1318.
Link:http://endo.endojournals.org/content/early/2008/12/18/en.2008-1318.full.pdf

4. Title: Islet Endothelial Cells and Pancreatic
-Cell

Proliferation: Studies in Vitro and during Pregnancy in Adult Rats.

Publication:Published online before print January 26, 2006, doi:10.1210/en.2005-0997 Endocrinology May 1, 2006vol. 147no. 52315-2324.

Link:http://endo.endojournals.org/content/147/5/2315.full.pdf+html