Acrp30 Human, His
Catalogue number
CYT-433
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Introduction
Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
脂联素,也称为Acrp30、AdipoQ和GBP-28,是最近发现的一种244个氨基酸的蛋白质,是apM1基因的产物,具有生理活性,在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族; 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体,它们是血清中循环的高阶复合物的组成部分。
Description
The Acrp30 Human is created as a recombinant protein with N-terminal fusion of His Tag. The Adiponectin His-Tagged Fusion Protein, produced in E. coli, is 26.4 kDa protein containing 230 amino acid residues of the Acrp30 Human and 12 additional amino acid residues – HisTag .
Acrp30 Human 是一种重组蛋白,其 N 端融合了 His 标签。 在大肠杆菌中产生的脂联素 His-Tagged 融合蛋白是 26.4 kDa 蛋白,含有 Acrp30 Human 的 230 个氨基酸残基和 12 个额外的氨基酸残基 – HisTag。
Source
Escherichia Coli.
Physical Appearance
Filtered White lyophilized powder.
Formulation
Acrp30 Human was filtered and lyophilized from 0.5 mg/ml in 0.02M Tris buffer pH7.5, 0.15M NaCl.
Solubility
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液,让冻干颗粒完全溶解。 产品不是无菌的! 在用于细胞培养之前,请用适当的无菌过滤器过滤产品。
Stability
Store lyophilized Acrp30 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的 Acrp30 Human 储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组 Acrp30 Human 可在 4°C 下储存一段有限的时间; 在 4°C 下两周后它没有显示任何变化。
Amino acid sequence
MRGSHHHHHHGSGHDQETTT QGPGVLLPLP KGACTGWMAG IPGHPGHNGA PGRDGRDGTP GEKGEKGDPG LIGPKGDIGE TGVPGAEGPR GFPGIQGRKG EPGEGAYVYR SAFSVGLETY VTIPNMPIRF TKIFYNQQNH YDGSTGKFHC NIPGLYYFAY HITVYMKDVK VSLFKKDKAM LFTYDQYQEN NVDQASGSVL LHLEVGDQVW LQVYGEGERN GLYADNDNDS TFTGFLLYHD TN.
Purity
Acrp30 Human purity is greater than 95% as determined by -PAGE.
Usage
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。
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