ProSpec-ANGPTL4 Human, HEK/CYT-698

ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.

The fasting-induced adipose factor was identified as an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. At the protein level, in human and mouse blood plasma, FIAF was found to be present both as a native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated FIAF, whereas in human white adipose tissue and SGBS adipocytes, only the native FIAF could be detected. Interestingly, the truncated FIAF was produced by human liver.
Experimental data suggest that FIAF is mainly presented in human blood plasma in a truncated form , whose level is increased by fenofibrate treatment. Levels of both truncated and native FIAF showed marked inter individual variation but were not associated with body mass index and were not influenced by prolonged semistarvation.

禁食诱导的脂肪因子被确定为通过禁食、过氧化物酶体增殖物激活受体激动剂和缺氧上调的脂肪细胞因子。 在蛋白质水平，在人和小鼠血浆中，发现 FIAF 以天然蛋白质和截短形式存在。 小鼠 3T3-L1 脂肪细胞的分化与截短 FIAF 的产生有关，而在人类白色脂肪组织和 SGBS 脂肪细胞中，只能检测到天然 FIAF。 有趣的是，截断的 FIAF 是由人类肝脏产生的。
实验数据表明，FIAF主要以截短形式存在于人血浆中，其水平因非诺贝特治疗而增加。 截断和原生 FIAF 的水平显示出明显的个体差异，但与体重指数无关，也不受长期半饥饿的影响。

The ANGPTL4 Human Recombinant is manufactured with C-terminal fusion of 11 amino acid FLAG Tag.
The ANGPTL4 Flag -Tagged Fusion Protein is a 44.2kDa protein containing 392 amino acid residues of the Angiopoietin-like Protein 4 and 11 additional amino acid residues – Flag Tag .

ANGPTL4 Human Recombinant 由 11 个氨基酸 FLAG 标签的 C 端融合制成。
ANGPTL4 Flag-Tagged 融合蛋白是一种 44.2kDa 的蛋白质，含有血管生成素样蛋白 4 的 392 个氨基酸残基和 11 个额外的氨基酸残基 – Flag Tag。

HEK293.

Filtered White lyophilized powder.

Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.

Add pyrogen free water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

加入无热原水至 0.5mg/ml 的工作浓度，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Store lyophilized Angiopoietin-like Protein 4 Human recombinant at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干的血管生成素样蛋白 4 人重组体储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Greater than 90% as determined by -PAGE.

GPVQSKSPRF ASWDEMNVLA HGLLQLGQGL REHAERTRSQ LSALERRLSA CGSACQGTEG STDLPLAPES RVDPEVLHSL QTQLKAQNSR IQQLFHKVAQ QQRHLEKQHL RIQHLQSQFG LLDHKHLDHE VAKPARRKRL PEMAQPVDPA HNVSRLHRLP RDCQELFQVG ERQSGLFEIQ PQGSPPFLVN CKMTSDGGWT VIQRRHDGSV DFNRPWEAYK AGFGDPHGEF WLGLEKVHSI TGDRNSRLAV QLRDWDGNAE LLQFSVHLGG EDTAYSLQLT APVAGQLGAT TVPPSGLSVP FSTWDQDHDL RRDKNCAKSL SGGWWFGTCS HSNLNGQYFR SIPQQRQKLK KGIFWKTWRG RYYPLQATTM LIQPMAAEAA SAAADYKDDDDK.

Western blotting.

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