ProSpec-GDF5 Human/CYT-442,Growth Differentiation Factor-5 Human Recombinant

Cartilage-derived morphogenetic protein-1, CDMP-1, LAP4, SYNS2, GDF-5, Radotermin, CDMP1, GDF5, Growth differentiation factor 5, BMP-14.

GDF-5 is a member of the bone morphogenetic protein family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.

Growth Differentiation Factor 5 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 120 amino acids and having a total molecular mass of 27.4kDa. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys.
GDF5 is purified by proprietary chromatographic techniques.

GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。 该家族的成员是胚胎和成人组织中细胞生长和分化的调节剂。 该基因的突变与Hunter-Thompson型肢端发育不良有关； 短指，C型； 和软骨发育不良，Grebe型。 这些关联证实基因产物在骨骼发育中发挥作用。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The protein was lyophilized without any additives.

It is recommended to reconstitute the lyophilized Growth Differentiation Factor-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的生长分化因子 5 用不低于 100µg/ml 的无菌 18MΩ-cm H2O 复溶，然后可以进一步稀释成其他水溶液。

Lyophilized Growth Differentiation Factor 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Growth Differentiation Factor-5 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干生长分化因子 5 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，生长分化因子 5 应在 4°C 下储存 2-7 天，并在 -18°C 以下供将来使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

GDF-5 activity as determined by the induction of alkaline phosphatase activity in ATDC5 cells is typically 10-20ng/ml.

APSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard solution of GDF5 as a Reference Standard.

蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 1.15 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. 反相高效液相色谱分析，使用 GDF5 的标准溶液作为参考标准。

Title:Growth/differentiation Factor-5 Induces Osteogenic Differentiation of Human Ligamentum Flavum Cells through Activation of ERK1/2 and p38 MAPK.
Publication:Cell Physiol Biochem 2010;26:179-186
Link:http://content.karger.com/produktedb/produkte.asp?doi=320526

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。