ProSpec-CTF1 Human/CYT-944

CTF1, CT1, CT-1, Cardiophin 1, Cardiotrophin-1.

Cardiotrophin 1 is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.
CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins in cardiac cells.
Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide and angiotensinogen mRNA.
Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.

心肌营养素 1 是白细胞介素 6 超家族的 201 个氨基酸成员。它是通过其在心肌细胞中诱导肥大反应的能力来确定的。 CT-1 mRNA 水平在心肌细胞和心脏非心肌细胞中均存在。 CT 1 在正常成人肺中也大量检测到，并在胎儿和成人气道平滑肌细胞中表达。 CT 1 激活 gp130 依赖性信号传导并刺激 Janus 激酶/信号转导和转录途径激活因子，以转导心肌细胞中的肥大和细胞保护信号。
CT 1 还具有神经营养功能。在胚胎第 14 天和出生后第一周之间，CTF1 缺乏导致小鼠脊髓和脑干核中运动神经元细胞死亡增加。此外，CT-1 是一种肝细胞存活因子，可有效减少急性肝损伤动物模型中的肝细胞损伤。心肌营养因子 1 的表达在缺氧刺激后增强，当在模拟缺血之前或在模拟缺血后的复氧时添加时，它可以保护心肌细胞。 Cardiotrophin 1 可以诱导心脏细胞中保护性热休克蛋白的表达。
Cardiotrophin-1 增加 ANP、脑利钠肽和血管紧张素原 mRNA 的心室表达。
Cardiophin 1 水平在心力衰竭患者、扩张型心肌病患者、中/重度二尖瓣关闭不全、稳定和不稳定型心绞痛患者以及急性心肌梗死后显着升高。

Cardiotrophin-1 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 201 amino acids and having a molecular mass of 21.2kDa.
The CTF1 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Cardiotrophin-1 Human Recombinant 是一条单一的、非糖基化的多肽链，含有 201 个氨基酸，分子量为 21.2kDa。
CTF1 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

CTF-1 protein was lyophilized from a 0.2µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 4mM HCl 中复溶冻干的 CTF1，然后可以进一步稀释成其他水溶液。

Lyophilized CTF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF-1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 CTF1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CTF-1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A.

The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0×10⁶ IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。