ProSpec-CTF1 Human, His/CYT-436

CTF1, CT1, CT-1, Cardiophin 1.

Cardiotrophin 1 is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.
CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins in cardiac cells.
Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide and angiotensinogen mRNA.
Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.

心肌营养素 1 是白细胞介素 6 超家族的 201 个氨基酸成员。它是通过其在心肌细胞中诱导肥大反应的能力来确定的。 CT-1 mRNA 水平在心肌细胞和心脏非心肌细胞中均存在。 CT 1 在正常成人肺中也大量检测到，并在胎儿和成人气道平滑肌细胞中表达。 CT 1 激活 gp130 依赖性信号传导并刺激 Janus 激酶/信号转导和转录途径激活因子，以转导心肌细胞中的肥大和细胞保护信号。
CT 1 还具有神经营养功能。在胚胎第 14 天和出生后第一周之间，CTF1 缺乏导致小鼠脊髓和脑干核中运动神经元细胞死亡增加。此外，CT-1 是一种肝细胞存活因子，可有效减少急性肝损伤动物模型中的肝细胞损伤。心肌营养因子 1 的表达在缺氧刺激后增强，当在模拟缺血之前或在模拟缺血后的复氧时添加时，它可以保护心肌细胞。 Cardiotrophin 1 可以诱导心脏细胞中保护性热休克蛋白的表达。
Cardiotrophin-1 增加 ANP、脑利钠肽和血管紧张素原 mRNA 的心室表达。
Cardiophin 1 水平在心力衰竭患者、扩张型心肌病患者、中/重度二尖瓣关闭不全、稳定和不稳定型心绞痛患者以及急性心肌梗死后显着升高。

The Cardiotrophin His-Tagged Fusion Protein Human, produced in E. coli, is 22.5 kDa protein containing 200 amino acid residues of the human Cardiotrophin and 12 additional amino acid residues – His Tag .

在大肠杆菌中产生的 Cardiotrophin His-Tagged Fusion Protein Human 是一种 22.5 kDa 的蛋白质，含有人 Cardiotrophin 的 200 个氨基酸残基和 12 个额外的氨基酸残基 – His 标签。

Escherichia Coli.

CTF1 was filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10?g/ml. In higher concentrations the solubility of this antigen is limited. Protein is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入 0.1M 醋酸盐缓冲液 pH-4 制备约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10?g/ml 的浓度。 在较高浓度下，该抗原的溶解度是有限的。 蛋白质不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

MRGSHHHHHH GSSRREGSLE DPQTDSSVSL LPHLEAKIRQ THSLAHLLTK YAEQLLQEYV QLQGDPFGLPSFSPPRLPVA GLSAPAPSHA GLPVHERLRL DAAALAALPP LLDAVCRRQA ELNPRAPRLL RRLEDAARQA RALGAAVEAL LAALGAANRG PRAEPPAATA SAASATGVFP AKVLGLRVCG LYREWLSRTE GDLGQLLPGG SA.

Purity of CTF1 Human Recombinant is greater than 90% as determined by -PAGE.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.