ProSpec-FASLG Human/CYT-031

Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL, CD178, FASLG, APT1LG1, CD95L, TNFSF6, ALPS1B.

The type II transmembrane protein FASLG is a member of the tumor necrosis factor superfamily. A fas ligand/receptor interaction has a significant part in the regulation of the immune system and the advancement of cancer. FASLG is expressed on the activated T cell surface as a nondisulfidelinked homotrimer. FASLG binding to Fas/CD95/TNFRSF6 on a nearby cell prompts apoptosis in the Fas expressing cell. FASLG is released from the cell surface by metalloproteinases as a soluble molecule that stays trimeric and is able to bind with Fas, but its capability to activate apoptosis is radically reduced. In addition, FASLG binds to DcR3 – a soluble trap receptor with no signal transduction capabilities. Flawed Fas-mediated apoptosis causes oncogenesis in addition to drug resistance in existing tumors. Constitutive expression of FASLG in a variety of tumors enables their immune evasion. Both mouse and human FASLG are active on mouse and human cells.

II型跨膜蛋白FASLG是肿瘤坏死因子超家族的成员。 fas 配体/受体相互作用在免疫系统调节和癌症进展中具有重要作用。 FASLG 在活化的 T 细胞表面以非二硫键同源三聚体的形式表达。 FASLG 与附近细胞上的 Fas/CD95/TNFRSF6 结合会促使表达 Fas 的细胞发生凋亡。 FASLG 作为可溶性分子通过金属蛋白酶从细胞表面释放，保持三聚体并能够与 Fas 结合，但其激活细胞凋亡的能力从根本上降低。 此外，FASLG 与 DcR3 结合 – 一种没有信号转导能力的可溶性陷阱受体。 除了现有肿瘤的耐药性外，有缺陷的 Fas 介导的细胞凋亡还会导致肿瘤发生。 FASLG 在多种肿瘤中的组成型表达使其能够逃避免疫。 小鼠和人类 FASLG 都对小鼠和人类细胞具有活性。

FASLG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids and having a molecular mass of 19.6kDa.
FASLG is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 FASLG Human Recombinant 是一条单一的、非糖基化的多肽链，包含 173 个氨基酸，分子量为 19.6kDa。
FASLG 在 N 末端与 21 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered colorless solution.

FASLG protein solution containing 20mM Tris-HCl buffer , 0.4M urea and 10% glycerol.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90% as determined by -PAGE.

MGSSHHHHHH SSGLVPRGSH MQIGHPSPPP EKKELRKVAH LTGKSNSRSM PLEWEDTYGI VLLSGVKYKK GGLVINETGL YFVYSKVYFR GQSCNNLPLS HKVYMRNSKY PQDLVMMEGK MMSYCTTGQM WARSSYLGAV FNLTSADHLY VNVSELSLVN FEESQTFFGL YKL.

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