ProSpec-DHH (C23II) Human/CYT-362

HHG-3, Desert Hedgehog homolog, MGC35145, Desert hedgehog protein, DHH.

DHH is part of the Hedgehog family which encodes signaling molecules that are involved in regulating morphogenesis. DHH protein is a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. Additionally, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in DHH protein have been associated with partial gonadal dysgenesis accompanied by minifascicular polyneuropathy. DHH plays a role both male gonadal differentiation and perineurial development.
DHH plays a role in intercellular signaling which is essential for a variety of patterning events during development. DHH functions as a spermatocyte survival factor in the testes & is essential for testes development.

DHH 是 Hedgehog 家族的一部分，它编码参与调节形态发生的信号分子。 DHH 蛋白是自催化切割的前体，N 端部分是可溶的并包含信号活性，而 C 端部分参与前体加工。 此外，C 末端产物将胆固醇部分共价连接到 N 末端产物，将 N 末端产物限制在细胞表面并防止其在整个生物体中自由扩散。 DHH 蛋白的缺陷与伴有小束状多发性神经病的部分性腺发育不全有关。 DHH 在雄性性腺分化和神经周围发育中均发挥作用。
DHH 在细胞间信号传导中发挥作用，这对于发育过程中的各种模式事件至关重要。 DHH 作为睾丸中的精母细胞存活因子，对睾丸发育至关重要。

DHH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 177 amino acids and having a molecular mass of 19.9kDa.
The DHH is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 DHH Human Recombinant 是一条单一的、非糖基化的多肽链，包含 177 个氨基酸，分子量为 19.9kDa。
DHH 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

It is recommended to reconstitute the lyophilized DHH in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 DHH，然后可以进一步稀释成其他水溶液。

Lyophilized DHH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DHH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG.

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The Biological Activity was determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 cells. The expected ED₅₀ for this effect is 15-45 μg/ml.

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