ProSpec-SHH Rat/CYT-1099

SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.

Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog helps in guiding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is necessary for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long-range Sonic HedgeHog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly.

重组人声波刺猬是一小部分分泌蛋白的一部分，这些蛋白对脊椎动物和无脊椎动物的发育都至关重要。 3个哺乳动物刺猬基因具有约60%的同源性。人类声波刺猬与小鼠基因有 99% 的同源性。 Sonic HedgeHog 有助于引导早期胚胎。它被认为是腹侧神经管、前后肢轴和腹侧体节的主要诱导信号。 Sonic HedgeHog 与与平滑结合的修补受体结合，以激活靶基因的转录。在没有声波 HedgeHog 的情况下，修补受体抑制了平滑的本构信号活性。 Sonic HedgeHog 还调节另一个因素，即 gli 癌基因。 Sonic HedgeHog 细胞间信号对于发育过程中的各种模式事件是必需的：由脊索产生的信号，它诱导神经管和体节中的腹侧细胞命运，以及用于形成发育中的肢芽前后轴模式的极化信号。 Sonic HedgeHog 表现出底板和运动神经元诱导活动。位于肢体区域 1 基因内含子的远程 Sonic HedgeHog 增强子的突变导致轴前多指畸形。

Sonic HedgeHog Recombinant Rat produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.9kDa.

SHH is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Sonic HedgeHog Recombinant Rat 是一条单一的、非糖基化的多肽链，包含 176 个氨基酸，分子量为 19.9kDa。

SHH 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

SHH is lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, pH 7.5.

It is recommended to reconstitute the lyophilized SHH in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 SHH，然后可以进一步稀释成其他水溶液。

Lyophilized Human Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

冻干的人声刺猬虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，Sonic HedgeHog 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Greater than 95.0% as determined by -PAGE.

MIIGPGRGFG KRQHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKARI HCSVKAENSV AAKSDG.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.