ProSpec-IFN g Mouse/CYT-358

Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I IFNs.

由特定抗原或有丝分裂原激活的淋巴细胞产生的 IFN-γ。
IFN-γ除具有抗病毒活性外，还具有重要的免疫调节功能，是巨噬细胞的强效激活剂，对转化细胞具有抗增殖作用，并能增强I型干扰素的抗病毒和抗肿瘤作用。

IFN-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 IFN-γ 小鼠重组体是一条单一的、非糖基化的多肽链，含有 134 个氨基酸，分子量为 15.6kDa。
IFN-γ 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 5% trehalose.

It is recommended to reconstitute the lyophilized IFN-gamma in sterile distilled water or 20mM AcOH at concentrations ranging between 0.1mg-0.5mg/ml, which can then be further diluted to other aqueous solutions.

建议在无菌蒸馏水或 20mM AcOH 中以 0.1mg-0.5mg/ml 的浓度重新配制冻干的 IFN-γ，然后可以将其进一步稀释成其他水溶液。

Lyophilized IFN-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 IFN-γ 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，IFN-γ 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.

The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 10⁷ Units/mg.

Title:IFN-γ and interleukin-4 reciprocally regulate CD8 expression in CD8⁺ T cells.
Publication:Published online before print November 6, 2008, doi: 10.1073/pnas.0809549105 PNAS November 11, 2008 vol. 105 no. 45 17475-17480
Link:http://www.pnas.org/content/105/45/17475.full

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.