ProSpec-IFNA1A Human/CYT-520

IFN-alpha 1a, IFN-a 1a, IFN alpha 1a.

At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

已知至少 23 种不同的 IFN-α 变体。 单个蛋白质的分子量在 19-26 kDa 之间，由长度为 156-166 和 172 个氨基酸的蛋白质组成。 所有 IFN-α 亚型在氨基酸位置 115-151 之间具有共同的保守序列区域，而氨基末端是可变的。 许多 IFN-α 亚型的序列仅在一个或两个位置上有所不同。 天然存在的变体还包括在羧基末端被 10 个氨基酸截短的蛋白质。

IFN-alpha 1a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 167 amino acids and having a molecular mass of 19.5kDa. The IFNA1A is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 IFN-α 1a 人重组体是一种单一的、非糖基化的多肽链，含有 167 个氨基酸，分子量为 19.5kDa。 IFNA1A 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The IFN-a 1a protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4, containing 3% Mannitol, 5% Trehalose, 0.05% Tween-80.

It is recommended to reconstitute the lyophilized IFN alpha 1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized IFNA1A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 1a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The specific activity as determined in a viral resistance assay viral ressistance assay was found to be 100,000,000IU/ mg.

MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNADSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.