ProSpec-IFNG Canine/CYT-404

Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

由特定抗原或有丝分裂原激活的淋巴细胞产生的 IFN-γ。
IFN-γ除具有抗病毒活性外，还具有重要的免疫调节功能，是巨噬细胞的强效激活剂，对转化细胞具有抗增殖作用，并能增强I型干扰素的抗病毒和抗肿瘤作用。

Recombinant IFNG Dog produced in E.coli cells is a non-glycosylated, homodimeric protein containing 143 amino acid chain and having a molecular mass of 16.9kDa. IFNG is purified by proprietary chromatographic techniques.

在大肠杆菌细胞中产生的重组 IFNG Dog 是一种非糖基化的同源二聚体蛋白，含有 143 个氨基酸链，分子量为 16.9kDa。 IFNG 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The IFNG was lyophilized from a 0.2µm filtered concentrated solution in 25mM Sodium Succinate, pH 5.0, 60mM NaCl, with 0.1% Tween-80.

It is recommended to reconstitute the lyophilized IFNG in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100μg/ml 的无菌蒸馏水中复溶冻干的 IFNG，然后可以进一步稀释成其他水溶液。

Lyophilized IFNG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFNG should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK

The ED50 as determined by an anti-viral assay using A-72 canine fibroma cells infected with vesicular stomatitis virus is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.